Invitrogen

BRCA2 Monoclonal Antibody (234403)

1 Published Figure

2 References

View all (22) BRCA2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

BRCA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

BRCA2 Antibody (MA5-23942) in IHC (P)

BRCA2 in Human Breast Cancer Tissue. BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using BRCA2 Monoclonal Antibody (Product # MA5-23942) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG HRP Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval. Tissue was stained u... View More

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Product Details

MA5-23942

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5-25 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

234403

Immunogen

E. coli-derived recombinant human BRCA2 Met1-Thr200

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2608610

Product Specific Information

This antibody detects endogenous human BRCA2.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Breast Cancer Type 2 susceptibility protein (BRCA2) is a tumor-suppressor protein reported to be involved in double-strand break repair, homologous recombination and S-phase checkpoint activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Olaparib-Resistant BRCA2^MUT Ovarian Cancer Cells with Restored BRCA2 Abrogate Olaparib-Induced DNA Damage and G2/M Arrest Controlled by the ATR/CHK1 Pathway for Survival.

Cells

Biegała Ł,Gajek A,Marczak A,Rogalska A

MA5-23942 was used in Western Blotting to elucidate olaparib resistance and enhance the efficacy of olaparib and examine intracellular factors exploited by OC cells to achieve decreased sensitivity to PARPi.

Wed Mar 29 00:00:00 UTC 2023

Olaparib-Resistant BRCA2^MUT Ovarian Cancer Cells with Restored BRCA2 Abrogate Olaparib-Induced DNA Damage and G2/M Arrest Controlled by the ATR/CHK1 Pathway for Survival.

Cells

Biegała Ł,Gajek A,Marczak A,Rogalska A

Wed Mar 29 00:00:00 UTC 2023

Loss of MAP3K7 Sensitizes Prostate Cancer Cells to CDK1/2 Inhibition and DNA Damage by Disrupting Homologous Recombination.

Molecular cancer research : MCR

Washino S,Rider LC,Romero L,Jillson LK,Affandi T,Ohm AM,Lam ET,Reyland ME,Costello JC,Cramer SD

MA5-23942 was used in Immunocytochemistry-immunoflourescence to demonstrate that loss of MAP3K7 is a main contributing factor to drug response through disruption of HR in prostate cancer.

Tue Oct 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: brca 2; brca-2; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset; breast and ovarian cancer susceptibility protein 2; breast cancer 2 tumor suppressor; breast cancer 2, early onset; Breast cancer type 2 susceptibility protein; FANCB; Fanconi anemia group D1 protein; mutant BRCA2; RP11-298P3.4

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Gene Aliases: BRCA2; BRCC2; BROVCA2; FACD; FAD; FAD1; FANCD; FANCD1; GLM3; PNCA2; XRCC11

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UniProt ID: (Human) P51587

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Entrez Gene ID: (Human) 675

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Function(s)

protease binding single-stranded DNA binding histone acetyltransferase activity protein binding H3 histone acetyltransferase activity H4 histone acetyltransferase activity gamma-tubulin binding

Process(es)

telomere maintenance via recombination double-strand break repair via homologous recombination DNA synthesis involved in DNA repair strand displacement cytokinesis oocyte maturation inner cell mass cell proliferation nucleotide-excision repair double-strand break repair DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator male meiosis I spermatogenesis brain development cell aging response to nutrient female gonad development response to X-ray response to UV-C response to gamma radiation hemopoiesis mammary gland development chromosome breakage homologous chromosome orientation involved in meiotic metaphase I plate congression response to estradiol regulation of cytokinesis negative regulation of mammary gland epithelial cell proliferation multicellular organism growth intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator histone H3 acetylation histone H4 acetylation positive regulation of transcription, DNA-templated positive regulation of mitotic cell cycle replication fork protection centrosome duplication establishment of protein localization to telomere mitotic recombination-dependent replication fork processing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BRCA2 Monoclonal Antibody (234403)

BRCA2 Antibody (MA5-23942) in IHC (P)

Product Details

MA5-23942

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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