Invitrogen

BRCC3 Polyclonal Antibody

BRCC3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

BRCC3 Antibody (PA5-20426) in ICC/IF

Immunofluorescence of BRCC36 in Human Brain cells with BRCC3 Polyclonal Antibody (Product # PA5-20426) at 20 µg/mL.

BRCC3 Antibody in Immunohistochemistry (IHC)

BRCC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BRCC3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

BRCC3 Antibody — Depletion of BRCC3 results in increased sensitivity to ionizing radiation. a Knockdown of BRCC3 was assessed by western-blotting (nc: negative control siRNA; si1: siRNA1 target BRCC3; si2: siRNA2 target BRCC3). b HNE1 and CNE2R cells were transfected with siRNAs, 24 h later they were seeded to 6-well plates and irradiated with different doses. Clones were stained and counted after 7-14 days. c Dose-survival curves of irradiated HNE1 and CNE2R cells (range, 0-6Gy, SF: survival fractions, SER: radiation sensitization enhancement ratio). In HNE1 cells SER of si1 and si2 was 1.26 (p < 0.05) and 1.21 (p < 0.05) separately; In CNE2R cells SER of si1 and si2 was 1.46 (p < 0.01) and 1.12 (p < 0.05) separately. d Immunofluorescent staining of γH2AX. HNE1 and CNE2R cells transfected with BRCC3 or control siRNA was exposed to 2Gy irradiation, and immunofluorescent staining of γH2AX foci was conducted before and 0.5 h, 6 h, 12 h, 24 h after irradiation (e) Quantification of γH2AX foci formation. A positive cell was determined by the presence of >10 foci/cell. The percentage of positive cells was compared by t-test. The asterisks indicate a significant (*p < 0.05,**p < 0.01) difference {KD}

Product Details

PA5-20426

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

2.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

20 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide from near the amino terminus of human BRCC36.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154606

Product Specific Information

A suggested positive control is MCF7 cell lysate.

PA5-20426 can be used with blocking peptide PEP-0543.

Target Information

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial DNA oxidation induces imbalanced activity of NLRP3/NLRP6 inflammasomes by activation of caspase-8 and BRCC36 in dry eye.

Journal of autoimmunity

Chi W,Hua X,Chen X,Bian F,Yuan X,Zhang L,Wang X,Chen D,Deng R,Li Z,Liu Y,de Paiva CS,Pflugfelder SC,Li DQ

Published figure using BRCC3 polyclonal antibody (Product # PA5-20426) in Immunofluorescence

Thu Jun 01 00:00:00 UTC 2017

Mitochondrial DNA oxidation induces imbalanced activity of NLRP3/NLRP6 inflammasomes by activation of caspase-8 and BRCC36 in dry eye.

Journal of autoimmunity

Chi W,Hua X,Chen X,Bian F,Yuan X,Zhang L,Wang X,Chen D,Deng R,Li Z,Liu Y,de Paiva CS,Pflugfelder SC,Li DQ

Published figure using BRCC3 polyclonal antibody (Product # PA5-20426) in Immunofluorescence

Thu Jun 01 00:00:00 UTC 2017

RPA3 is a potential marker of prognosis and radioresistance for nasopharyngeal carcinoma.

Journal of cellular and molecular medicine

Qu C,Zhao Y,Feng G,Chen C,Tao Y,Zhou S,Liu S,Chang H,Zeng M,Xia Y

PA5-20426 was used in Immunohistochemistry on paraffin embedded tissues to demonstrate that RPA3 can regulate the radiosensitivity and DNA repair capacity of nasopharyngeal carcinoma cells, providing a potential marker for prognosis.

Wed Nov 01 00:00:00 UTC 2017

BRCC3 acts as a prognostic marker in nasopharyngeal carcinoma patients treated with radiotherapy and mediates radiation resistance in vitro.

Radiation oncology (London, England)

Tu Z,Xu B,Qu C,Tao Y,Chen C,Hua W,Feng G,Chang H,Liu Z,Li G,Jiang C,Yi W,Zeng M,Xia Y

PA5-20426 was used in immunohistochemistry - paraffin section and western blot to study the role of BRCC3 in nasopharyngeal carcinoma prognosis and radiosensitivity

Sat May 30 00:00:00 UTC 2015

BRCC3 acts as a prognostic marker in nasopharyngeal carcinoma patients treated with radiotherapy and mediates radiation resistance in vitro.

Radiation oncology (London, England)

Tu Z,Xu B,Qu C,Tao Y,Chen C,Hua W,Feng G,Chang H,Liu Z,Li G,Jiang C,Yi W,Zeng M,Xia Y

PA5-20426 was used in immunohistochemistry - paraffin section and western blot to study the role of BRCC3 in nasopharyngeal carcinoma prognosis and radiosensitivity

Sat May 30 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 6.1A protein; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; Lys-63-specific deubiquitinase BRCC36; RP11-143H17.2

View more View less

Gene Aliases: BRCC3; BRCC36; C6.1A; CXorf53

View more View less

UniProt ID: (Human) P46736, (Rat) B2RYM5, (Mouse) P46737

View more View less

Entrez Gene ID: (Human) 79184, (Rat) 316794, (Mouse) 210766

View more View less

Function(s)

thiol-dependent ubiquitin-specific protease activity protein binding metallopeptidase activity enzyme regulator activity polyubiquitin binding metal ion binding peptidase activity hydrolase activity pyroglutamyl-peptidase activity

Process(es)

double-strand break repair proteolysis response to X-ray response to ionizing radiation positive regulation of DNA repair regulation of catalytic activity protein K63-linked deubiquitination histone H2A K63-linked deubiquitination DNA repair cellular response to DNA damage stimulus chromatin modification G2 DNA damage checkpoint double-strand break repair via nonhomologous end joining mitotic nuclear division cell division

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

BRCC3 Polyclonal Antibody

BRCC3 Antibody (PA5-20426) in ICC/IF

Product Details

PA5-20426

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!