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BRD7 Antibody (PA5-78484) in ICC/IF
BRD7 Polyclonal Antibody detects BRD7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD7 protein stained by BRD7 Polyclonal Antibody (Product # PA5-78484) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78484
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human BRD7. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735535
Product Specific Information
Positive Control: HeLa, HeLa nuclear extract
Predicted Reactivity: Mouse (85%), Rat (84%), Bovine (88%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BRD7 acts both as coactivator and as corepressor. For example, it activates the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. In addition, it is required for TP53-mediated cell-cycle arrest in response to oncogene activation. BRD7 promotes acetylation of TP53 at Lys-382, and thereby promotes efficient recruitment of TP53 to target promoters, inhibiting cell cycle progression from G1 to S phase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 75 kDa bromodomain protein; Bromodomain-containing protein 7; Protein CELTIX-1
Gene Aliases: BP75; BRD7; CELTIX1; NAG4
UniProt ID: (Human) Q9NPI1
Entrez Gene ID: (Human) 29117
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell cycle
negative regulation of cell proliferation
Wnt signaling pathway
positive regulation of histone acetylation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
regulation of signal transduction by p53 class mediator
negative regulation of G1/S transition of mitotic cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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