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BRF2 Antibody (PA5-97155) in WB
Western blot analysis of BRF2 in extracts of various cell lines. Samples were incubated with BRF2 Polyclonal antibody (Product # PA5-97155) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-97155
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 180-419 of human BRF2 (NP_0607802)
Conjugate
Form
Liquid
Concentration
1.62 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808957
Product Specific Information
Immunogen sequence: FKLFQASPSV PAKYVEDKEK MLSRTMQLVE LANETWLVTG RHPLPVITAA TFLAWQSLQP ADRLSCSLAR FCKLANVDLP YPASSRLQEL LAVLLRMAEQ LAWLRVLRLD KRSVVKHIGD LLQHRQSLVR SAFRDGTAEV ETREKEPPGW GQGQGEGEVG NNSLGLPQGK RPASPALLLP PCMLKSPKRI CPVPPVSTVT GDENISDSEI EQYLRTPQEV RDFQRAQAAR QAATSVPNPP
Positive Samples: DU145, Mouse lung; Cellular Location: Nucleus
Target Information
This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-related factor 2; BRF-2; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like; FLJ11052; hBRFU; hTFIIIB50; RNA polymerase III transcription initiation factor BRFU; TFIIIB50; transcription factor IIB- related factor, TFIIIB50; Transcription factor IIIB 50 kDa subunit
Gene Aliases: 2700059M06Rik; 5730512K07Rik; AU018504; AU022602; BRF2; BRFU; PRO1470; TFIIIB50
UniProt ID: (Human) Q9HAW0, (Rat) Q4V8D6, (Mouse) Q3UAW9
Entrez Gene ID: (Human) 55290, (Rat) 306542, (Mouse) 66653
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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