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Product Details
OSB00076W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide to from aa region 660-710 of human BRSK2 conjugated to an immunogenic carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: BRSK2.
Target Information
BRSK2 is a brain-selective serine/threonine kinase 2 that is mainly expressed in the brain, with weaker expression in testis and pancreas. BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhanced endogenous BRSK2 kinase activity in a caffeine-sensitive manner and caused translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BR serine/threonine-protein kinase 2; brain serine/threonine kinase 2; Brain-selective kinase 2; Brain-specific serine/threonine-protein kinase 2; protein kinase SAD1B; SAD1B; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase BRSK2; Serine/threonine-protein kinase SAD-A; testicular tissue protein Li 30
Gene Aliases: 4833424K13Rik; BRSK2; C11orf7; HUSSY-12; Kiaa4256; PEN11B; SAD-A; SAD1; SADA; STK29
UniProt ID: (Human) Q8IWQ3, (Mouse) Q69Z98
Entrez Gene ID: (Human) 9024, (Mouse) 75770, (Rat) 293631
magnesium ion binding
protein serine/threonine kinase activity
ATP binding
protein kinase binding
tau-protein kinase activity
ATPase binding
ATPase regulator activity
nucleotide binding
protein kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
G2/M transition of mitotic cell cycle
protein phosphorylation
exocytosis
apoptotic process
cell cycle
mitotic nuclear division
nervous system development
axonogenesis
phosphorylation
peptidyl-serine phosphorylation
establishment of cell polarity
neuron differentiation
actin cytoskeleton reorganization
intracellular signal transduction
neuron projection morphogenesis
cell division
regulation of insulin secretion involved in cellular response to glucose stimulus
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to DNA damage stimulus
response to UV
response to ionizing radiation
ERAD pathway
regulation of ATPase activity
regulation of retrograde protein transport, ER to cytosol
Performance Guarantee
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