Invitrogen

BTRC Monoclonal Antibody (1B1D2)

BTRC Antibody in Immunocytochemistry (ICC/IF)

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BTRC Antibody (37-3400) in ICC/IF

Immunofluorescent analysis of BTRC was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BTRC (1B1D2) Mouse Monoclonal Antibody (Product # 37-3400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

BTRC Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

BTRC Antibody in Western Blot, Immunoprecipitation (WB, IP)

BTRC Antibody in Western Blot, ChIP Assay (WB, ChIP)

BTRC Antibody in Western Blot, Flow Cytometry (WB, Flow)

Product Image Gallery

BTRC Antibody — Figure 5 NNK enhances hnRNP-U/betaTrCP translocation to the cytoplasm and induces DNMT1 accumulation in the nucleus, but this effect is attenuated by AKT inhibition. ( A ) Immunofluorescence confocal microscopy to localize DNMT1 (green), betaTrCP (red), and nucleus (blue; DAPI). The merged figures showed colocalization of DNMT1 with betaTrCP protein in A549 cells treated with DMSO control. Mutually exclusive localization of DNMT1 and betaTrCP was observed in cells treated with NNK. Original magnification, x630. Scale bar: 20 mum. ( B ) Cell fractionation assay to analyze distribution of nuclear and cytoplasmic proteins, using anti-DNMT1, anti-betaTrCP, anti-hnRNP-U, and anti-GSK3beta Ser9 antibodies. Anti-GAPDH and anti-lamin A/C antibodies were used as cytoplasmic and nuclear protein markers, respectively. The distribution of betaTrCP and hnRNP-U proteins was contrary to that of DNMT1 protein. ( C ) DNMT1 and betaTrCP interaction was interrupted by NNK treatment. In contrast, betaTrCP and hnRNP interaction was increased by NNK treatment. ( D ) Cell fractionation assay was performed in A549 cells with or without AKT siRNA. Inhibition of AKT induced both hnRNP-U and betaTrCP proteins predominantly located in the nucleus, where DNMT1 protein level decreased. ( E ) IP assay using anti-AKT antibody showed that hnRNP formed a complex with AKT, and the interaction was attenuated by LY294002. ( F ) An increase of hnRNP-U phosphorylation was mediated by exogenous AKT, which increased {TM}

Product Details

37-3400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 6 publications 6 publications

ChIP assay (ChIP)

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1B1D2

Immunogen

Recombinant human beta-TrCP fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533313

Target Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

APE1 redox function is required for activation of Yes-associated protein 1 under reflux conditions in Barrett's-associated esophageal adenocarcinomas.

Journal of experimental & clinical cancer research : CR

Ballout F,Lu H,Chen L,Sriramajayam K,Que J,Meng Z,Wang TC,Giordano S,Zaika A,McDonald O,Peng D,El-Rifai W

37-3400 was used in Proximity Ligation Assay (PLA) to establish a novel function of APE1 in EAC progression elucidating druggable molecular vulnerabilities via targeting APE1 or YAP1 for the treatment of EAC.

Thu Sep 01 00:00:00 UTC 2022

ZAKα Recognizes Stalled Ribosomes through Partially Redundant Sensor Domains.

Molecular cell

Vind AC,Snieckute G,Blasius M,Tiedje C,Krogh N,Bekker-Jensen DB,Andersen KL,Nordgaard C,Tollenaere MAX,Lund AH,Olsen JV,Nielsen H,Bekker-Jensen S

37-3400 was used in Western Blot to investigate if long isoform of ZAK (ZAKα) directly associates with ribosomes by inserting its flexible C terminus into the ribosomal intersubunit space.

Thu May 21 00:00:00 UTC 2020

Long noncoding RNA ASB16-AS1 inhibits adrenocortical carcinoma cell growth by promoting ubiquitination of RNA-binding protein HuR.

Cell death & disease

Long B,Yang X,Xu X,Li X,Xu X,Zhang X,Zhang S

37-3400 was used in Western Blotting to prove that lncRNA ASB16-AS1 was down-regulated in adrenocortical carcinoma and ASB16-AS1 functions as tumor suppressor in vitro and in vivo.

Fri Nov 20 00:00:00 UTC 2020

Long noncoding RNA ASB16-AS1 inhibits adrenocortical carcinoma cell growth by promoting ubiquitination of RNA-binding protein HuR.

Cell death & disease

Long B,Yang X,Xu X,Li X,Xu X,Zhang X,Zhang S

37-3400 was used in Western Blotting to prove that lncRNA ASB16-AS1 was down-regulated in adrenocortical carcinoma and ASB16-AS1 functions as tumor suppressor in vitro and in vivo.

Fri Nov 20 00:00:00 UTC 2020

Long noncoding RNA ASB16-AS1 inhibits adrenocortical carcinoma cell growth by promoting ubiquitination of RNA-binding protein HuR.

Cell death & disease

Long B,Yang X,Xu X,Li X,Xu X,Zhang X,Zhang S

37-3400 was used in Western Blotting to prove that lncRNA ASB16-AS1 was down-regulated in adrenocortical carcinoma and ASB16-AS1 functions as tumor suppressor in vitro and in vivo.

Fri Nov 20 00:00:00 UTC 2020

Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer.

Nucleic acids research

Lan Y,Xiao X,He Z,Luo Y,Wu C,Li L,Song X

Wed Jun 20 00:00:00 UTC 2018

Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer.

Nucleic acids research

Lan Y,Xiao X,He Z,Luo Y,Wu C,Li L,Song X

Wed Jun 20 00:00:00 UTC 2018

TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells.

Cancer cell

Diamantopoulou Z,White G,Fadlullah MZH,Dreger M,Pickering K,Maltas J,Ashton G,MacLeod R,Baillie GS,Kouskoff V,Lacaud G,Murray GI,Sansom OJ,Hurlstone AFL,Malliri A

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Western Blot

Mon May 08 00:00:00 UTC 2017

Hippo signaling interactions with Wnt/β-catenin and Notch signaling repress liver tumorigenesis.

The Journal of clinical investigation

Kim W,Khan SK,Gvozdenovic-Jeremic J,Kim Y,Dahlman J,Kim H,Park O,Ishitani T,Jho EH,Gao B,Yang Y

373400 was used in western blot to demonstrate that the Hippo, Wnt/beta-catenin, and Notch pathways interact to regulate liver size and inhibit hepatocellular carcinoma

Tue Jan 03 00:00:00 UTC 2017

Interaction of tankyrase and peroxiredoxin II is indispensable for the survival of colorectal cancer cells.

Nature communications

Kang DH,Lee DJ,Lee S,Lee SY,Jun Y,Kim Y,Kim Y,Lee JS,Lee DK,Lee S,Jho EH,Yu DY,Kang SW

37-3400 was used in Western Blotting to demonstrate that peroxiredoxin type II plays a tumour-promoting role in colorectal cancer by interacting with a poly(ADP-ribose) polymerase tankyrase.

Wed Jun 28 00:00:00 UTC 2017

Substrate trapping proteomics reveals targets of the βTrCP2/FBXW11 ubiquitin ligase.

Molecular and cellular biology

Kim TY,Siesser PF,Rossman KL,Goldfarb D,Mackinnon K,Yan F,Yi X,MacCoss MJ,Moon RT,Der CJ,Major MB

37-3400 was used in western blot to use the parallel adapter capture proteomics approach to identify substrates of betaTrCP2/FBXW11.

Thu Jan 01 00:00:00 UTC 2015

Growth-arrest-specific 7C protein inhibits tumor metastasis via the N-WASP/FAK/F-actin and hnRNP U/β-TrCP/β-catenin pathways in lung cancer.

Oncotarget

Tseng RC,Chang JW,Mao JS,Tsai CD,Wu PC,Lin CJ,Lu YL,Liao SY,Cheng HC,Hsu HS,Wang YC

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Immunoprecipitation

Tue Dec 29 00:00:00 UTC 2015

Growth-arrest-specific 7C protein inhibits tumor metastasis via the N-WASP/FAK/F-actin and hnRNP U/β-TrCP/β-catenin pathways in lung cancer.

Oncotarget

Tseng RC,Chang JW,Mao JS,Tsai CD,Wu PC,Lin CJ,Lu YL,Liao SY,Cheng HC,Hsu HS,Wang YC

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Immunoprecipitation

Tue Dec 29 00:00:00 UTC 2015

RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression.

Molecular and cellular biology

Ram RR,Mendiratta S,Bodemann BO,Torres MJ,Eskiocak U,White MA

37-3400 was used in western blot to study the involvement of RASSF1A inactivation in carcinogenesis

Sun Jun 01 00:00:00 UTC 2014

PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation.

PloS one

Wang D,Zhang P,Gao K,Tang Y,Jin X,Zhang Y,Yi Q,Wang C,Yu L

37-3400 was used in western blot to investigate the role of Rap1GAP in the cell cycle regulation.

Tue Jul 28 00:00:00 UTC 2015

β-Transducin repeat-containing protein 1 (β-TrCP1)-mediated silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) protein degradation promotes tumor necrosis factor α (TNFα)-induced inflammatory gene expression.

The Journal of biological chemistry

Hsu KS,Kao HY

Fri Aug 30 00:00:00 UTC 2013

The Journal of biological chemistry

Hsu KS,Kao HY

Fri Aug 30 00:00:00 UTC 2013

The Journal of biological chemistry

Hsu KS,Kao HY

Fri Aug 30 00:00:00 UTC 2013

The Journal of biological chemistry

Hsu KS,Kao HY

Fri Aug 30 00:00:00 UTC 2013

FAM123A binds to microtubules and inhibits the guanine nucleotide exchange factor ARHGEF2 to decrease actomyosin contractility.

Science signaling

Siesser PF,Motolese M,Walker MP,Goldfarb D,Gewain K,Yan F,Kulikauskas RM,Chien AJ,Wordeman L,Major MB

37-3400 was used in western blot to study the localization and function of FAM23 family members.

Tue Sep 04 00:00:00 UTC 2012

mTOR drives its own activation via SCF(βTrCP)-dependent degradation of the mTOR inhibitor DEPTOR.

Molecular cell

Gao D,Inuzuka H,Tan MK,Fukushima H,Locasale JW,Liu P,Wan L,Zhai B,Chin YR,Shaik S,Lyssiotis CA,Gygi SP,Toker A,Cantley LC,Asara JM,Harper JW,Wei W

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Western Blot

Fri Oct 21 00:00:00 UTC 2011

FOXO3a-Dependent Mechanism of E1A-Induced Chemosensitization.

Cancer research

Su JL,Cheng X,Yamaguchi H,Chang YW,Hou CF,Lee DF,Ko HW,Hua KT,Wang YN,Hsiao M,Chen PB,Hsu JM,Bast RC,Hortobagyi GN,Hung MC

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Western Blot

Tue Nov 01 00:00:00 UTC 2011

FOXO3a-Dependent Mechanism of E1A-Induced Chemosensitization.

Cancer research

Su JL,Cheng X,Yamaguchi H,Chang YW,Hou CF,Lee DF,Ko HW,Hua KT,Wang YN,Hsiao M,Chen PB,Hsu JM,Bast RC,Hortobagyi GN,Hung MC

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Western Blot

Tue Nov 01 00:00:00 UTC 2011

SCF/{beta}-TrCP promotes glycogen synthase kinase 3-dependent degradation of the Nrf2 transcription factor in a Keap1-independent manner.

Molecular and cellular biology

Rada P,Rojo AI,Chowdhry S,McMahon M,Hayes JD,Cuadrado A

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Immunoprecipitation

Tue Mar 01 00:00:00 UTC 2011

SCF/{beta}-TrCP promotes glycogen synthase kinase 3-dependent degradation of the Nrf2 transcription factor in a Keap1-independent manner.

Molecular and cellular biology

Rada P,Rojo AI,Chowdhry S,McMahon M,Hayes JD,Cuadrado A

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Immunoprecipitation

Tue Mar 01 00:00:00 UTC 2011

Preservations of nephrin and synaptopodin by recombinant hepatocyte growth factor in podocytes for the attenuations of foot process injury and albuminuria in nephritic mice.

Nephrology (Carlton, Vic.)

Kato T,Mizuno S,Nakamura T

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Western Blot

Tue Mar 01 00:00:00 UTC 2011

The tobacco-specific carcinogen NNK induces DNA methyltransferase 1 accumulation and tumor suppressor gene hypermethylation in mice and lung cancer patients.

The Journal of clinical investigation

Lin RK,Hsieh YS,Lin P,Hsu HS,Chen CY,Tang YA,Lee CF,Wang YC

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Western Blot

Mon Feb 01 00:00:00 UTC 2010

βTrCP-dependent degradation of CDC25B phosphatase at the metaphase-anaphase transition is a pre-requisite for correct mitotic exit.

Cell cycle (Georgetown, Tex.)

Thomas Y,Coux O,Baldin V

37-3400 was used in Immunoprecipitation to demonstrate that, in mammalian cells, βTrCp participates to CDC25B turnover, and is required for the complete degradation of CDC25B at the metaphase-anaphase transition.

Mon Nov 01 00:00:00 UTC 2010

SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation.

Nature

Westbrook TF,Hu G,Ang XL,Mulligan P,Pavlova NN,Liang A,Leng Y,Maehr R,Shi Y,Harper JW,Elledge SJ

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Flow Cytometry

Thu Mar 20 00:00:00 UTC 2008

SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation.

Nature

Westbrook TF,Hu G,Ang XL,Mulligan P,Pavlova NN,Liang A,Leng Y,Maehr R,Shi Y,Harper JW,Elledge SJ

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Flow Cytometry

Thu Mar 20 00:00:00 UTC 2008

SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation.

Nature

Westbrook TF,Hu G,Ang XL,Mulligan P,Pavlova NN,Liang A,Leng Y,Maehr R,Shi Y,Harper JW,Elledge SJ

Published figure using BTRC monoclonal antibody (Product # 37-3400) in Flow Cytometry

Thu Mar 20 00:00:00 UTC 2008

TAZ promotes PC2 degradation through a SCFbeta-Trcp E3 ligase complex.

Molecular and cellular biology

Tian Y,Kolb R,Hong JH,Carroll J,Li D,You J,Bronson R,Yaffe MB,Zhou J,Benjamin T

37-3400 was used in western blot to use knockout mice and zebrafish to study the contribution of TAZ to polycystic kidney disease

Sat Sep 01 00:00:00 UTC 2007

Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress.

Molecular cell

Mailand N,Bekker-Jensen S,Bartek J,Lukas J

37-3400 was used in Western Blotting to suggest Claspin degradation facilitates timely reversal of the checkpoint response and delineates the period permissive for Chk1 activation during cell cycle progression.

Fri Aug 04 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: beta-transducin repeat containing protein; beta-TrCP1; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; F-box and WD-repeat protein 1B; F-box/WD repeat-containing protein 1A; pIkappaBalpha-E3 receptor subunit

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Gene Aliases: BETA-TRCP; beta-TrCP1; betaTrCP; BTRC; BTRCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

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UniProt ID: (Human) Q9Y297

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Entrez Gene ID: (Human) 8945, (Rat) 361765

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Function(s)

ubiquitin-protein transferase activity protein binding beta-catenin binding ligase activity protein phosphorylated amino acid binding protein dimerization activity ubiquitin protein ligase activity

Process(es)

G2/M transition of mitotic cell cycle protein polyubiquitination stimulatory C-type lectin receptor signaling pathway protein dephosphorylation ubiquitin-dependent protein catabolic process signal transduction viral process Wnt signaling pathway protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization mammary gland epithelial cell proliferation NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway regulation of circadian rhythm positive regulation of circadian rhythm regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of proteolysis negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated rhythmic process T cell receptor signaling pathway stress-activated MAPK cascade positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition branching involved in mammary gland duct morphogenesis regulation of canonical Wnt signaling pathway regulation of proteasomal protein catabolic process cellular response to organic cyclic compound protein catabolic process regulation of cell cycle

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BTRC Monoclonal Antibody (1B1D2)

BTRC Antibody (37-3400) in ICC/IF

Product Details

37-3400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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