OriGene

BTRC Monoclonal Antibody (OTI2H2), TrueMAB™

View all (25) BTRC antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF502465

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI2H2

Immunogen

Human recombinant protein fragment corresponding to amino acids 52-354 of human BTRC produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-TrCP1; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; F-box and WD-repeat protein 1B; F-box/WD repeat-containing protein 1A; pIkappaBalpha-E3 receptor subunit

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Gene Aliases: BETA-TRCP; betaTrCP; BTRC; BTRCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

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UniProt ID: (Human) Q9Y297

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Entrez Gene ID: (Human) 8945

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Function(s)
ubiquitin-protein transferase activity protein binding beta-catenin binding ligase activity protein phosphorylated amino acid binding protein dimerization activity ubiquitin protein ligase activity
Process(es)
G2/M transition of mitotic cell cycle protein polyubiquitination stimulatory C-type lectin receptor signaling pathway protein dephosphorylation ubiquitin-dependent protein catabolic process signal transduction viral process Wnt signaling pathway protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization mammary gland epithelial cell proliferation NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway regulation of circadian rhythm positive regulation of circadian rhythm regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of proteolysis negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated rhythmic process T cell receptor signaling pathway stress-activated MAPK cascade positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition branching involved in mammary gland duct morphogenesis regulation of canonical Wnt signaling pathway regulation of proteasomal protein catabolic process cellular response to organic cyclic compound
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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