Proteintech

BUB1 Polyclonal Antibody

BUB1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

BUB1 Antibody (13330-1-AP) in ICC/IF

Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

13330-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BUB1 Fusion Protein Ag4138 (785-1083 aa encoded by BC028201)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KLVYVHHLL GEGAFAQVYE ATQGDLNDAK NKQKFVLKVQ KPANPWEFYI GTQLMERLKP SMQHMFMKFY SAHLFQNGSV LVGELYSYGT LLNAINLYKN TPEKVMPQGL VISFAMRMLY MIEQVHDCEI IHGDIKPDNF ILGNGFLEQD DEDDLSAGLA LIDLGQSIDM KLFPKGTIFT AKCETSGFQC VEMLSNKPWN YQIDYFGVAA TVYCMLFGTY MKVKNEGGEC KPEGLFRRLP HLDMWNEFFH VMLNIPDCHH LPSLDLLRQK LKKVFQQHYT NKIRALRNRL IVLLLECKRS (785-1083 aa encoded by BC028201)

Target Information

BUB1A is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma.

Thoracic cancer

Chen R,Wang Z,Lu T,Liu Y,Ji Y,Yu Y,Tou F,Guo S

Sat Apr 01 00:00:00 UTC 2023

Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma.

Thoracic cancer

Chen R,Wang Z,Lu T,Liu Y,Ji Y,Yu Y,Tou F,Guo S

Sat Apr 01 00:00:00 UTC 2023

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Frontiers in pharmacology

Mengyan X,Kun D,Xinming J,Yutian W,Yongqian S

Sat Oct 01 00:00:00 UTC 2022

Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.

Journal of experimental & clinical cancer research : CR

Chen L,Song Y,Hou T,Li X,Cheng L,Li Y,Xing Y

Fri Jun 03 00:00:00 UTC 2022

A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.

Frontiers in bioengineering and biotechnology

Xu W,Mo Y,He Y,Fan Y,He G,Fu W,Chen S,Liu J,Liu W,Peng L,Xiao Y

Tue Feb 02 00:00:00 UTC 2021

Stathmin guides personalized therapy in oral squamous cell carcinoma.

Cancer science

Ju WT,Ma HL,Zhao TC,Liang SY,Zhu DW,Wang LZ,Li J,Zhang ZY,Zhou G,Zhong LP

Wed Apr 01 00:00:00 UTC 2020

Stathmin guides personalized therapy in oral squamous cell carcinoma.

Cancer science

Ju WT,Ma HL,Zhao TC,Liang SY,Zhu DW,Wang LZ,Li J,Zhang ZY,Zhou G,Zhong LP

Wed Apr 01 00:00:00 UTC 2020

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Nucleic acids research

Yuan F,Jin X,Li D,Song Y,Zhang N,Yang X,Wang L,Zhu WG,Tian C,Zhao Y

Thu Sep 05 00:00:00 UTC 2019

High expression of CDK1 and BUB1 predicts poor prognosis of pancreatic ductal adenocarcinoma.

Gene

Piao J,Zhu L,Sun J,Li N,Dong B,Yang Y,Chen L

Sat Jun 15 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; budding uninhibited by benzimidazoles 1 homolog; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase

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Gene Aliases: BUB1; BUB1A; BUB1L; hBUB1

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UniProt ID: (Human) O43683

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Entrez Gene ID: (Human) 699, (Dog) 100682599

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding

Process(es)

protein phosphorylation apoptotic process sister chromatid cohesion regulation of sister chromatid cohesion mitotic nuclear division mitotic cell cycle checkpoint mitotic spindle assembly checkpoint cell proliferation viral process cell division regulation of chromosome segregation spindle assembly checkpoint

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BUB1 Polyclonal Antibody

BUB1 Antibody (13330-1-AP) in ICC/IF

Product Details

13330-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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