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Rockland
Bacteriophage M13 Polyclonal Antibody
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Bacteriophage M13 Antibody (600-401-MR5) in ELISA
ELISA results of purified polyclonal Rabbit Bacteriophage M13 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Coating Buffer pH9.5, Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) at 1:30,000 and substrate (p/n TMBE-1000).
Product Details
600-401-MR5
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-M13 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of Bacteriophage M13 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against Tail virion protein G7P. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence with Enterobacteria phage M13, Enterobacteria phage f1, and Enterobacteria phage fd.
Target Information
Tail virion protein G7P (Bacteriophage M13) may initiate with G9P the virion concomitant assembly-budding process, by interacting with the packaging signal of the viral genome. The assembly-budding takes place at the host inner membrane. In turn, G7P and G9P are present at the end of the filamentous virion that emerges first from the bacterial host. Anti-Bacteriophage M13 Antibody is useful for researchers interested in HCP, Host Cell Membranes, Transmembrane Viruses, and E. coli bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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