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Invitrogen
Band 3 Monoclonal Antibody (5G2G7)
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FIGURE: 1 / 3
Band 3 Antibody (MA5-49186) in IHC (P)
Immunohistochemistry analysis of Band 3 in paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with Band 3 Monoclonal antibody (Product # MA5-49186) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG w... View More
Product Details
MA5-49186
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
5G2G7
Immunogen
E.coli-derived human SLC4A1 (Position: E28-N365).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074844
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human SLC4A1 shares 75.7% and 74.5% amino acid (aa) sequence identity with and rat SLC4A1, respectively.
Positive Control - WB: human K562 whole cell. IHC: human spleen tissue. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The Band 3 protein is the most abundant integral protein of human erythrocyte membranes and functions as the major anion transporter polypeptide of erythrocytes. Band 3 is a 90-100 kDa protein, its microheterogeneity is ascribed mainly to the structural heterogeneity of the carbohydrate moiety. The C-terminal portion of the molecule spans the membrane several times, and is responsible for anion transport. The N-terminal portion, comprising almost half of the polypeptide chain of Band 3, is located inside the red cell and interacts with cytoskeletal and cytoplasmic proteins such as ankyrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AE 1; Anion exchange protein 1; anion exchanger 1; anion exchanger-1; Band 3 anion transport protein; CAMP; CD233; Diego blood group; erythrocyte membrane protein band 3; erythroid anion exchange protein; Froese blood group; MGC116750; MGC116753; MGC126619; MGC126623; solute carrier family 4 (anion exchanger), member 1 (Diego blood group); Solute carrier family 4 member 1; solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group); solute carrier family 4, anion exchanger, number 1; Swann blood group; Waldner blood group; Wright blood group; ZNF828
Gene Aliases: AE1; BND3; CD233; CHC; DI; EMPB3; EPB3; FR; RTA1A; SAO; SLC4A1; SPH4; SW; WD; WD1; WR
UniProt ID: (Human) P02730
Entrez Gene ID: (Human) 6521
actin binding
inorganic anion exchanger activity
protein binding
protein C-terminus binding
anion transmembrane transporter activity
bicarbonate transmembrane transporter activity
chloride transmembrane transporter activity
anion:anion antiporter activity
enzyme binding
ankyrin binding
protein homodimerization activity
protein anchor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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