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Exalpha
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Positive control: MCF-7 cell line.
This antibody can be used for Western blotting (0.5-1 µg/mL), immunohistochemistry on formalin/paraffain embedded tissues (1-2 µg/mL) and immunoprecipitation (2 µg/mg of protein lysate). Antibody reacts with mutant as well as wild-type p53 protein.
Members of the Bcl-2 Family (including Bcl-2, Bcl-XLand Bax) play an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, Bax is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of Bax and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis regulator BAX; Apoptosis regulator Bax cytoplasmic isoform beta; Bax beta; BaxB; BCL2 associated X protein; BCL2 associated X protein isoform beta
Gene Aliases: Bax
UniProt ID: (Mouse) Q07813
Entrez Gene ID: (Mouse) 12028
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