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Invitrogen
Bestrophin 1 Polyclonal Antibody
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FIGURE: 1 / 1
Bestrophin 1 Antibody (PA5-91898) in WB
Western blot analysis of Bestrophin 1 in extracts of various cell lines. Samples were incubated with Bestrophin 1 Polyclonal antibody (Product # PA5-91898) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-91898
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 171-270 of human BEST1 (NP_0041741)
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806320
Product Specific Information
Positive Samples: BT-474, 293T, Mouse testis, Mouse brain, Rat testis, Rat brain; Cellular Location: Basolateral cell membrane, Cell membrane, Multi-pass membrane protein
Immunogen sequence: LEKLSLPHNM FWVPWVWFAN LSMKAWLGGR IRDPILLQSL LNEMNTLRTQ CGHLYAYDWI SIPLVYTQVV TVAVYSFFLT CLVGRQFLNP AKAYPGHELD
Target Information
The retinal pigment epithelium (RPE) and choroid represent a differentiated system of the eye that sustains normal retinal health and function. Best vitelliform macular dystrophy, known as Best disease, is an early-onset autosomal dominant condition in which accumulation of lipofuscin-like material within and beneath the RPE leads to progressive loss of central vision. The lipofuscin-like material in the macular area appears as a yellow mass like the yolk of an egg that later becomes darker and irregular in color, a process known as "scrambling the egg". Best disease is frequently a reflection of mutations in the Bestrophin gene, which encodes a protein containing four putative transmembrane domains and localizes to the basolateral plasma membrane of RPE cells. Human Bestrophin forms oligomeric chloride channels that are sensitive to intracellular calcium. Missense mutations at the Bestrophin locus reduces or abolishes Bestrophin protein mediated membrane current. The human Bestrophin gene encodes a 585 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BEST 1; Best disease; best macular dystrophy; Best1V1Delta2; Bestrophin-1; Bestrophin1; TU15B; vitelliform macular dystrophy (Best disease, bestrophin); vitelliform macular dystrophy 2 homolog; Vitelliform macular dystrophy protein 2; Vitelliform macular dystrophy protein 2 homolog; VMD 2
Gene Aliases: ARB; BEST; BEST1; BMD; Bmd1; mBest1; RP50; TU15B; VMD2
UniProt ID: (Human) O76090, (Mouse) O88870
Entrez Gene ID: (Human) 7439, (Mouse) 24115, (Rat) 293735
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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