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Invitrogen
Bi-1 Polyclonal Antibody
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FIGURE: 1 / 7
Bi-1 Antibody (PA5-143940) in ICC/IF
Immunocytochemistry analysis of Bi-1 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Bi-1 Polyclonal antibody (Product # PA5-143940) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Product Details
PA5-143940
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human BI-1/TMBIM6 (RKINFDALLKFSHITPSTQQHLK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075154
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human RT4 whole cell, human CACO-2 whole cell. IHC: human endometrial adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue. ICC/IF: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress-causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, itis thought to involve regulation of Ca2+ handling by the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bax inhibitor 1; BI-1; testis enhanced gene transcript; Testis-enhanced gene transcript protein; Transmembrane BAX inhibitor motif-containing protein 6
Gene Aliases: BAXI1; BI-1; BI1; TEGT; TMBIM6
UniProt ID: (Human) P55061
Entrez Gene ID: (Human) 7009
autophagy
response to unfolded protein
spermatogenesis
lung development
negative regulation of protein binding
endoplasmic reticulum calcium ion homeostasis
negative regulation of apoptotic process
negative regulation of immunoglobulin secretion
negative regulation of endoribonuclease activity
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
response to L-glutamate
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of mRNA endonucleolytic cleavage involved in unfolded protein response
negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
negative regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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