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Invitrogen
Blimp-1 Monoclonal Antibody (3H2-E8), DyLight™ 550
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Product Details
MA5-16117
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3H2-E8
Immunogen
A fragment of the mouse Blimp-1 (residues 199-409).
Conjugate
Excitation/Emission Max
556/570 nm
View spectra
Form
Liquid
Concentration
0.69 mg/mL
Purification
Protein G
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152755
Product Specific Information
In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form).
Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues.
This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining.
Suggested positive control: P3X (murine plasmocytoma) cell lysates.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; RP1-134E15.1
Gene Aliases: b2b1765Clo; Blimp-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1
UniProt ID: (Human) O75626, (Mouse) Q60636
Entrez Gene ID: (Human) 639, (Mouse) 12142
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
methyltransferase activity
histone deacetylase binding
metal ion binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
morphogenesis of a branching structure
maternal placenta development
heart valve development
ventricular septum development
transcription, DNA-templated
germ cell development
post-embryonic development
positive regulation of gene expression
negative regulation of B cell proliferation
negative regulation of lipopolysaccharide-mediated signaling pathway
methylation
regulation of natural killer cell differentiation
regulation of extrathymic T cell differentiation
aorta development
eye photoreceptor cell development
cell fate commitment
positive regulation of B cell differentiation
artery morphogenesis
regulation of NK T cell differentiation
intestinal epithelial cell development
trophoblast giant cell differentiation
coronary vasculature development
sebum secreting cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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