Invitrogen

Blimp-1 Monoclonal Antibody (6D3), eBioscience™

Blimp-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 32

Blimp-1 Antibody (14-5963-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 µg/mL of Anti-Human/Mouse Blimp1 Purified or 10 µg/mL of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) followed by Anti-Rat IgG Biotin, and DAB visualization.

Blimp-1 Antibody in Flow Cytometry (Flow)

Blimp-1 Antibody in Immunocytochemistry (ICC/IF)

Blimp-1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Blimp-1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

Blimp-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Blimp-1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Blimp-1 Antibody in Immunohistochemistry (IHC)

Blimp-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

14-5963-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

(0.5 µg)/test)

View 1 publication 1 publication

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6D3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1907437

Product Specific Information

Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection.

Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections.

Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

14-5963-82 was used in Immunohistochemistry (Paraffin) to show that depletion of MCPIP1 in macrophages and granulocytes (Mcpip1fl/fl-LysMcre+ C57BL/6 mice) is sufficient to trigger severe autoimmune disease.

Thu Mar 18 00:00:00 UTC 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

Thu Mar 18 00:00:00 UTC 2021

Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.

Disease models & mechanisms

Dobosz E,Lorenz G,Ribeiro A,Würf V,Wadowska M,Kotlinowski J,Schmaderer C,Potempa J,Fu M,Koziel J,Lech M

Thu Mar 18 00:00:00 UTC 2021

MEF2D sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity.

The Journal of clinical investigation

Di Giorgio E,Wang L,Xiong Y,Akimova T,Christensen LM,Han R,Samanta A,Trevisanut M,Bhatti TR,Beier UH,Hancock WW

14-5963 was used in Western Blotting to study the role played by MEF2D in sustaining effector Foxp3+ Treg functions without abrogating their basal actions suggests its suitability for drug discovery efforts in cancer therapy.

Tue Dec 01 00:00:00 UTC 2020

Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.

Theranostics

Ge W,Tan SJ,Wang SH,Li L,Sun XF,Shen W,Wang X

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunohistochemistry

Mon May 03 00:00:00 UTC 2021

Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis.

Nature communications

Oulès B,Philippeos C,Segal J,Tihy M,Vietri Rudan M,Cujba AM,Grange PA,Quist S,Natsuga K,Deschamps L,Dupin N,Donati G,Watt FM

14-5963-82 was used in Immunohistochemistry (Paraffin) to show that GATA6, which is expressed in the upper pilosebaceous unit of normal human skin, is down-regulated in acne.

Tue Oct 20 00:00:00 UTC 2020

Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale.

PeerJ

Wang X,Liao T,Wan C,Yang X,Zhao J,Fu R,Yao Z,Huang Y,Shi Y,Chang G,Zheng Y,Luo F,Liu Z,Wang Y,Mao X,Zhao XY

14-5963-82 was used in Immunocytochemistry to demonstrate that the 3D MC induction system combined with low-cell attachment plates facilitated the generation of hPGCLCs at a large scale.

Thu Mar 31 00:00:00 UTC 2022

Principles of early human development and germ cell program from conserved model systems.

Nature

Kobayashi T,Zhang H,Tang WWC,Irie N,Withey S,Klisch D,Sybirna A,Dietmann S,Contreras DA,Webb R,Allegrucci C,Alberio R,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

Principles of early human development and germ cell program from conserved model systems.

Nature

Kobayashi T,Zhang H,Tang WWC,Irie N,Withey S,Klisch D,Sybirna A,Dietmann S,Contreras DA,Webb R,Allegrucci C,Alberio R,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

Principles of early human development and germ cell program from conserved model systems.

Nature

Kobayashi T,Zhang H,Tang WWC,Irie N,Withey S,Klisch D,Sybirna A,Dietmann S,Contreras DA,Webb R,Allegrucci C,Alberio R,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

The aryl hydrocarbon receptor controls cell-fate decisions in B cells.

The Journal of experimental medicine

Vaidyanathan B,Chaudhry A,Yewdell WT,Angeletti D,Yen WF,Wheatley AK,Bradfield CA,McDermott AB,Yewdell JW,Rudensky AY,Chaudhuri J

14-5963 was used in Western Blotting to show that the aryl hydrocarbon receptor is highly induced upon B cell activation and serves a critical role in regulating activation-induced cell fate outcomes.

Sun Jan 01 00:00:00 UTC 2017

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

14-5963 was used in Immunofluorescence to investigate the role of nanog in unipotent primordial germ cells in mice.

Thu Jan 21 00:00:00 UTC 2016

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

14-5963 was used in Immunofluorescence to investigate the role of nanog in unipotent primordial germ cells in mice.

Thu Jan 21 00:00:00 UTC 2016

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunofluorescence

Thu Jan 15 00:00:00 UTC 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunofluorescence

Thu Jan 15 00:00:00 UTC 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunofluorescence

Thu Jan 15 00:00:00 UTC 2015

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Immunofluorescence

Thu Jan 15 00:00:00 UTC 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using Blimp-1 monoclonal antibody (Product # 14-5963-82) in Western Blot

Wed Apr 01 00:00:00 UTC 2015

Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.

PLoS genetics

Mould AW,Morgan MA,Nelson AC,Bikoff EK,Robertson EJ

14-5963 was used in Immunohistochemistry to demonstrate that Blimp1/Prdm1 is pivotal in the postnatal metabolic switch that occurs in the small intestine.

Wed Jul 01 00:00:00 UTC 2015

Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.

PLoS genetics

Mould AW,Morgan MA,Nelson AC,Bikoff EK,Robertson EJ

14-5963 was used in Immunohistochemistry to demonstrate that Blimp1/Prdm1 is pivotal in the postnatal metabolic switch that occurs in the small intestine.

Wed Jul 01 00:00:00 UTC 2015

Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.

PLoS genetics

Mould AW,Morgan MA,Nelson AC,Bikoff EK,Robertson EJ

14-5963 was used in Immunohistochemistry to demonstrate that Blimp1/Prdm1 is pivotal in the postnatal metabolic switch that occurs in the small intestine.

Wed Jul 01 00:00:00 UTC 2015

Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.

Journal of immunology (Baltimore, Md. : 1950)

Park KS,Bayles I,Szlachta-McGinn A,Paul J,Boiko J,Santos P,Liu J,Wang Z,Borghesi L,Milcarek C

14-5963 was used in Western Blotting to define the effects of the transcription elongation factor eleven nineteen lysine-rich leukaemia gene.

Sat Nov 01 00:00:00 UTC 2014

Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses.

Journal of immunology (Baltimore, Md. : 1950)

White CA,Pone EJ,Lam T,Tat C,Hayama KL,Li G,Zan H,Casali P

14-5963 was used in Western Blotting to study the epigenetic mechanisms which modulate expression of proteins involved in B cell differentiation and autoimmunity..

Mon Dec 15 00:00:00 UTC 2014

BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.

Stem cell reports

Kretzschmar K,Cottle DL,Donati G,Chiang MF,Quist SR,Gollnick HP,Natsuga K,Lin KI,Watt FM

Tue Oct 14 00:00:00 UTC 2014

Distinct fibroblast lineages determine dermal architecture in skin development and repair.

Nature

Driskell RR,Lichtenberger BM,Hoste E,Kretzschmar K,Simons BD,Charalambous M,Ferron SR,Herault Y,Pavlovic G,Ferguson-Smith AC,Watt FM

14-5963 was used in Immunohistochemistry to explain why wounding is linked to formation of ECM-rich scar tissue that lacks hair follicles.

Thu Dec 12 00:00:00 UTC 2013

Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation.

EMBO reports

Grabole N,Tischler J,Hackett JA,Kim S,Tang F,Leitch HG,Magnúsdóttir E,Surani MA

Mon Jul 01 00:00:00 UTC 2013

Sox2 modulates the function of two distinct cell lineages in mouse skin.

Developmental biology

Lesko MH,Driskell RR,Kretzschmar K,Goldie SJ,Watt FM

14-5963 was used in Immunohistochemistry to investigate how Sox2 modulates the function of two distinct cell lineages in mouse skin.

Tue Oct 01 00:00:00 UTC 2013

A genome-wide regulatory network identifies key transcription factors for memory CD8⁺ T-cell development.

Nature communications

Hu G,Chen J

14-5963 was used in Western Blotting to investigate the transcriptional control of memory CD8+ T-cell development.

Tue Jul 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; RP1-134E15.1

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Gene Aliases: b2b1765Clo; Blimp-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1

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UniProt ID: (Human) O75626, (Mouse) Q60636

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Entrez Gene ID: (Human) 639, (Mouse) 12142

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding methyltransferase activity histone deacetylase binding metal ion binding promoter-specific chromatin binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter morphogenesis of a branching structure maternal placenta development heart valve development ventricular septum development transcription, DNA-templated germ cell development post-embryonic development positive regulation of gene expression negative regulation of B cell proliferation negative regulation of lipopolysaccharide-mediated signaling pathway methylation regulation of natural killer cell differentiation regulation of extrathymic T cell differentiation aorta development eye photoreceptor cell development cell fate commitment positive regulation of B cell differentiation artery morphogenesis regulation of NK T cell differentiation intestinal epithelial cell development trophoblast giant cell differentiation coronary vasculature development sebum secreting cell proliferation

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Blimp-1 Monoclonal Antibody (6D3), eBioscience™

Blimp-1 Antibody (14-5963-82) in IHC (P)

Product Details

14-5963-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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