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Reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Chicken - Dog + Goat ++ Guinea Pig ± Horse + Human + Monkey + Mouse - Rabbit - Rat ± Sheep ++ Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Albumin; bovine albumin; BSA; MGC179277; serum albumin
Gene Aliases: ALB; BOS_6773
UniProt ID: (Bovine) P02769
Entrez Gene ID: (Bovine) 280717
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