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          • Primary Antibodies ›
          • BrdU Antibodies

          Proteintech

          BrdU Monoclonal Antibody (1B10E12)

          63 References
          View all (134) BrdU antibodies

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          Cite BrdU Monoclonal Antibody (1B10E12)

          • Antibody Testing Data (6)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
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          BrdU Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          BrdU Antibody (66241-1-IG) in ICC/IF

          Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (Product # 66241-1-IG, Clone: 1B10E12 ) at dilution of 1:1,000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BrdU Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BrdU Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Product Details

          66241-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 11 publications 11 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:150-1:600
          View 32 publications 32 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications

          Not applicable (N/A)

          -
          View 14 publications 14 publications
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1B10E12

          Immunogen

          Compound

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.55 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody does not cross react with Thymidine.

          Target Information

          Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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