Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • BrdU Antibodies

          Invitrogen

          BrdU Monoclonal Antibody (BU-1)

          Advanced Verification
          9 Published Figures
          50 References
          View all (134) BrdU antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite BrdU Monoclonal Antibody (BU-1)

          • Antibody Testing Data (4)
          • Published Figures (9)
          • Advanced Verification (1)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          BrdU Antibody (MA3-071) in ICC/IF

          Immunofluorescent analysis of BrdU (green) showing staining in the nucleus of Hela cells treated with 100nM BrdU (right) and untreated Hela cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BrdU monoclonal antibody (Product # MA3-071) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunohistochemistry (IHC)
          BrdU Antibody in Flow Cytometry (Flow)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunohistochemistry (Frozen) (IHC (F))
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunocytochemistry (ICC/IF)
          BrdU Antibody in Immunohistochemistry (IHC)
          BrdU Antibody in Immunohistochemistry (IHC)
          BrdU Antibody in Immunohistochemistry - Free Floating (IHC (Free))
          BrdU Antibody

          Product Details

          MA3-071

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          1:1,000-1:2,500
          View 23 publications 23 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunohistochemistry - Free Floating (IHC (Free))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 16 publications 16 publications

          Flow Cytometry (Flow)

          1:100
          View 5 publications 5 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical, Chicken, Mouse, Non-human primate

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          BU-1

          Immunogen

          5-iodouridine covalently coupled to ovalbumin.

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • DyLight 488
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.05 mg/mL

          Purification

          Protein A

          Storage buffer

          0.01M HEPES with 0.15M NaCl, 30% glycerol

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10986341

          Product Specific Information

          MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples.

          MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications.

          The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin.

          This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

          Target Information

          Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-5d94444789-48zfn:80/100.66.135.186:80.
          git-commit: ec3573d8beaf0fd412f2d0ea2dcb8dd14c74098c
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0