Invitrogen

BrdU Monoclonal Antibody (BU20A), PE, eBioscience™

14 Published Figures

18 References

View all (134) BrdU antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (14)

FIGURE: 1 / 15

BrdU Antibody (12-5071-42) in Flow

Intracellular staining of 2-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes using the Foxp3/Transcription Staining Buffer Set (Product # 00-5523-00) with Anti-Mouse CD4 FITC (Product # 11-0042-82) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-BrdU PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-5071-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BU20A

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11149499

Product Specific Information

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment.

For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (Product # 00-5525) and protocol.

Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Staining Buffer Set (Product # 00-5523-00) or the BrdU Staining Buffer Set (Product # 00-5525-0) and protocol. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

DOT1L Is a Novel Cancer Stem Cell Target for Triple-Negative Breast Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Kurani H,Razavipour SF,Harikumar KB,Dunworth M,Ewald AJ,Nasir A,Pearson G,Van Booven D,Zhou Z,Azzam D,Wahlestedt C,Slingerland J

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Mon May 02 00:00:00 UTC 2022

Gain-of-function hot spot mutant p53^R248Q regulation of integrin/FAK/ERK signaling in esophageal squamous cell carcinoma.

Translational oncology

Yu VZ,So SS,Lung ML

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.

International journal of molecular sciences

Nakata S,Yuan M,Rubens JA,Kahlert UD,Maciaczyk J,Raabe EH,Eberhart CG

12-5071 was used in Flow cytometry/Cell sorting to introduce either wildtype BCOR or BCOR-ITD into human and murine neural stem cells and analyzed them with quantitative RT-PCR and RNA-sequencing, as well as growth, clonogenicity, and invasion assays.

Sat Apr 10 00:00:00 UTC 2021

BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.

International journal of molecular sciences

Nakata S,Yuan M,Rubens JA,Kahlert UD,Maciaczyk J,Raabe EH,Eberhart CG

Sat Apr 10 00:00:00 UTC 2021

Detecting chromatin interactions between and along sister chromatids with SisterC.

Nature methods

Oomen ME,Hedger AK,Watts JK,Dekker J

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration.

Cell research

Zhou S,Zhang W,Cai G,Ding Y,Wei C,Li S,Yang Y,Qin J,Liu D,Zhang H,Shao X,Wang J,Wang H,Yang W,Wang H,Chen S,Hu P,Sun L

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2020

ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin's lymphoma and testicular cancer cells.

Cell death & disease

Constantin D,Widmann C

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Mon Nov 30 00:00:00 UTC 2020

Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells.

Cell reports

Ramezani-Rad P,Chen C,Zhu Z,Rickert RC

12-5071-42 was used in Flow Cytometry to conclude that selected GC B cells in the light zone potentially require disengagement from BCR signaling to accumulate cyclin D3 and undergo clonal expansion in the DZ.

Tue Nov 17 00:00:00 UTC 2020

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Tue Jun 19 00:00:00 UTC 2018

FOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.

The Journal of clinical investigation

Li H,Liu P,Xu S,Li Y,Dekker JD,Li B,Fan Y,Zhang Z,Hong Y,Yang G,Tang T,Ren Y,Tucker HO,Yao Z,Guo X

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Mon Apr 03 00:00:00 UTC 2017

Adipose tissue macrophages develop from bone marrow-independent progenitors in Xenopus laevis and mouse.

Journal of leukocyte biology

Hassnain Waqas SF,Noble A,Hoang AC,Ampem G,Popp M,Strauß S,Guille M,Röszer T

12-5071 was used in Flow cytometry/Cell sorting to study adipose tissue macrophage ontogeny.

Fri Sep 01 00:00:00 UTC 2017

PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts.

Genes to cells : devoted to molecular & cellular mechanisms

Danno S,Kubouchi K,Mehruba M,Abe M,Natsume R,Sakimura K,Eguchi S,Oka M,Hirashima M,Yasuda H,Mukai H

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

Nature cell biology

Fong MY,Zhou W,Liu L,Alontaga AY,Chandra M,Ashby J,Chow A,O'Connor ST,Li S,Chin AR,Somlo G,Palomares M,Li Z,Tremblay JR,Tsuyada A,Sun G,Reid MA,Wu X,Swiderski P,Ren X,Shi Y,Kong M,Zhong W,Chen Y,Wang SE

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Sun Feb 01 00:00:00 UTC 2015

Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma.

eLife

Hettmer S,Schinzel AC,Tchessalova D,Schneider M,Parker CL,Bronson RT,Richards NG,Hahn WC,Wagers AJ

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Sat Oct 24 00:00:00 UTC 2015

CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.

Molecular biology of the cell

Ohta S,Wood L,Toramoto I,Yagyu K,Fukagawa T,Earnshaw WC

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Wed Apr 01 00:00:00 UTC 2015

Atm deletion with dual recombinase technology preferentially radiosensitizes tumor endothelium.

The Journal of clinical investigation

Moding EJ,Lee CL,Castle KD,Oh P,Mao L,Zha S,Min HD,Ma Y,Das S,Kirsch DG

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Fri Aug 01 00:00:00 UTC 2014

Interleukin-7 expression and its effect on natural killer cells in patients with multiple sclerosis.

Journal of neuroimmunology

Su N,Shi SX,Zhu X,Borazanci A,Shi FD,Gan Y

12-5071 was used in Flow cytometry/Cell sorting to investigate the biological function of interleukin-7 on natural killer cells in multiple sclerosis patients.

Sat Nov 15 00:00:00 UTC 2014

MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells.

Cell death & disease

Cao JX,Lu Y,Qi JJ,An GS,Mao ZB,Jia HT,Li SY,Ni JH

Published figure using BrdU monoclonal antibody (Product # 12-5071-42) in Flow Cytometry

Thu Sep 25 00:00:00 UTC 2014

Isoniazid induces apoptosis of activated CD4+ T cells: implications for post-therapy tuberculosis reactivation and reinfection.

The Journal of biological chemistry

Tousif S,Singh DK,Ahmad S,Moodley P,Bhattacharyya M,Van Kaer L,Das G

12-5071 was used in Flow cytometry/Cell sorting to show that Isoniazid treatment of Mycobacterium tuberculosis treatment is associated with immune impairment.

Fri Oct 31 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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BrdU Monoclonal Antibody (BU20A), PE, eBioscience™

BrdU Antibody (12-5071-42) in Flow

Product Details

12-5071-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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