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Invitrogen
BrdU Recombinant Rabbit Monoclonal Antibody (HL1111)
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FIGURE: 1 / 3
BrdU Antibody (MA5-46930) in ICC/IF
Immunofluorescent analysis of BrdU in mock and treated HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU monoclonal antibody (Product # MA5-46930) diluted at 1:2,000 and competitors antibody ([BU1/75 (ICR1)]) diluted at 1:2,000. Blue: Fluoroshield with DAPI.
Product Details
MA5-46930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1111
Immunogen
The immunogen used to generate this antibody corresponds to BrdU.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2938002
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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