Invitrogen

Btk Polyclonal Antibody

Advanced Verification
1 Published Figure
2 References
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-20085

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-2 µg/mL
-

Immunohistochemistry (IHC)

5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

ELISA (ELISA)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153734

Product Specific Information

A suggested positive control is U937 cell lysate.

PA5-20085 can be used with blocking peptide PEP-0203.

Target Information

Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily of tyrosine kinases. Mutations in the BTK gene have been linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; Kinase EMB; MGC126261; MGC126262; OTTHUMP00000023677; RP1-164F3.2; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); X-linked immune deficiency

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Gene Aliases: AGMX1; AI528679; AT; ATK; BPK; BTK; IMD1; PSCTK1; xid; XLA

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UniProt ID: (Human) Q06187, (Mouse) P35991

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Entrez Gene ID: (Human) 695, (Mouse) 12229

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Function(s)
protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding identical protein binding metal ion binding nucleotide binding protein kinase activity lipid binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
negative regulation of cytokine production adaptive immune response regulation of B cell cytokine production MyD88-dependent toll-like receptor signaling pathway regulation of B cell apoptotic process transcription, DNA-templated protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway I-kappaB kinase/NF-kappaB signaling mesoderm development peptidyl-tyrosine phosphorylation calcium-mediated signaling intracellular signal transduction peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway B cell activation regulation of cell proliferation innate immune response positive regulation of B cell differentiation cell maturation B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity apoptotic signaling pathway immune system process histamine secretion by mast cell regulation of transcription, DNA-templated apoptotic process response to organic substance phosphorylation cell differentiation cellular response to reactive oxygen species protein autophosphorylation cellular response to molecule of fungal origin
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