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Invitrogen
Bub1 Polyclonal Antibody
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FIGURE: 1 / 1
Bub1 Antibody (PA5-78687) in WB
Western blot analysis of BUB1 in, Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse thymus tissue lysates, Lane 11: mouse heart tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE... View More
Product Details
PA5-78687
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Bub1 recombinant protein (Position: V731-K1085).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745803
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human HEK293 whole cell, human Hela whole cell, human Jurkat whole cell, human HL-60 whole cell, rat testis tissue, rat heart tissue, rat PC-12 whole cell, mouse testis tissue, mouse thymus tissue, mouse heart tissue, mouse NIH/3T3 whole cell.
Target Information
BUB1A is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; budding uninhibited by benzimidazoles 1 homolog; hBUB1; mBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
Gene Aliases: AL022991; BUB1; BUB1A; BUB1L; C80208; D2Xrf87; hBUB1
UniProt ID: (Human) O43683, (Mouse) O08901
Entrez Gene ID: (Human) 699, (Rat) 296137, (Mouse) 12235
protein phosphorylation
chromosome segregation
regulation of sister chromatid cohesion
cell proliferation
regulation of chromosome segregation
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
sister chromatid cohesion
mitotic nuclear division
mitotic cell cycle checkpoint
mitotic spindle assembly checkpoint
viral process
cell division
spindle assembly checkpoint
cell cycle
embryo development
phosphorylation
mitotic spindle checkpoint
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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