Proteintech

BubR1 Polyclonal Antibody

BubR1 Antibody in Immunocytochemistry (ICC/IF)

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BubR1 Antibody (11504-2-AP) in ICC/IF

Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

BubR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11504-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BubR1 Fusion Protein Ag2053 (719-1050 aa encoded by BC018739)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QSPWCSQYR RQLLKSLPEL SASAELCIED RPMPKLEIEK EIELGNEDYC IKREYLICED YKLFWVAPRN SAELTVIKVS SQPVPWDFYI NLKLKERLNE DFDHFCSCYQ YQDGCIVWHQ YINCFTLQDL LQHSEYITHE ITVLIIYNLL TIVEMLHKAE IVHGDLSPRC LILRNRIHDP YDCNKNNQAL KIVDFSYSVD LRVQLDVFTL SGFRTVQILE GQKILANCSS PYQVDLFGIA DLAHLLLFKE HLQVFWDGSF WKLSQNISEL KDGELWNKFF VRILNANDEA TVSVLGELAA EMNGVFDTTF QSHLNKALWK VGKLTSPGAL LFQ (719-1050 aa encoded by B C018739)

Target Information

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Revealing active components and action mechanism of Fritillariae Bulbus against non-small cell lung cancer through spectrum-effect relationship and proteomics.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Liu FJ,Chen XY,Yang J,Zhao Z,Wang QL,Li P,Jiang Y,Li HJ

Wed Feb 01 00:00:00 UTC 2023

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Frontiers in pharmacology

Mengyan X,Kun D,Xinming J,Yutian W,Yongqian S

Sat Oct 01 00:00:00 UTC 2022

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.

Nature communications

Zhou CJ,Wang XY,Dong YH,Wang DH,Han Z,Zhang XJ,Sun QY,Carroll J,Liang CG

Tue Dec 13 00:00:00 UTC 2022

Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors.

Bioorganic chemistry

Liang Y,Zhang M,Zhou P,Liu M,Li J,Wang Y

Fri Oct 01 00:00:00 UTC 2021

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.

International journal of biological sciences

Xu W,Huang M,Guo J,Zhang H,Wang D,Liu T,Liu H,Chen S,Gao P,Mu K

Mon Sep 27 00:00:00 UTC 2021

Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy.

Technology in cancer research & treatment

Zhang YP,Bao ZW,Wu JB,Chen YH,Chen JR,Xie HY,Zhou L,Wu J,Zheng SS

Mon Nov 29 00:00:00 UTC 2021

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Nucleic acids research

Yuan F,Jin X,Li D,Song Y,Zhang N,Yang X,Wang L,Zhu WG,Tian C,Zhao Y

Thu Sep 05 00:00:00 UTC 2019

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Oncogene

Zhu ZC,Liu JW,Li K,Zheng J,Xiong ZQ

Tue May 01 00:00:00 UTC 2018

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Oncogene

Zhu ZC,Liu JW,Li K,Zheng J,Xiong ZQ

Tue May 01 00:00:00 UTC 2018

Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.

European journal of medicinal chemistry

Zhou P,Liang Y,Zhang H,Jiang H,Feng K,Xu P,Wang J,Wang X,Ding K,Luo C,Liu M,Wang Y

Sat Jan 20 00:00:00 UTC 2018

Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.

European journal of medicinal chemistry

Zhou P,Liang Y,Zhang H,Jiang H,Feng K,Xu P,Wang J,Wang X,Ding K,Luo C,Liu M,Wang Y

Sat Jan 20 00:00:00 UTC 2018

Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.

Cancer letters

Wen C,Chen J,Zhang D,Wang H,Che J,Qin Q,He L,Cai Z,Lin M,Lou Q,Huang L,Chen D,Iwamoto A,Ren D,Wang L,Lan P,Wang J,Liu H,Yang X

Wed Dec 28 00:00:00 UTC 2016

Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.

Cancer letters

Wen C,Chen J,Zhang D,Wang H,Che J,Qin Q,He L,Cai Z,Lin M,Lou Q,Huang L,Chen D,Iwamoto A,Ren D,Wang L,Lan P,Wang J,Liu H,Yang X

Wed Dec 28 00:00:00 UTC 2016

Protein phosphatase 4 plays dual roles during cell proliferation.

Cell proliferation

Huang X,Liu J,Shen T,Meng X,Dou L,Lin Y,Li J

Fri Apr 01 00:00:00 UTC 2016

Protein phosphatase 4 plays dual roles during cell proliferation.

Cell proliferation

Huang X,Liu J,Shen T,Meng X,Dou L,Lin Y,Li J

Fri Apr 01 00:00:00 UTC 2016

SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation.

Molecular reproduction and development

Yuan YF,Zhai R,Liu XM,Khan HA,Zhen YH,Huo LJ

Fri Aug 01 00:00:00 UTC 2014

SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation.

Molecular reproduction and development

Yuan YF,Zhai R,Liu XM,Khan HA,Zhen YH,Huo LJ

Fri Aug 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: BUB1B, mitotic checkpoint serine/threonine kinase; BubR1; budding uninhibited by benzimidazoles 1 beta; budding uninhibited by benzimidazoles 1 homolog beta; hBub1; hBUBR1; MAD3/BUB1-related protein kinase; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Protein SSK1

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Gene Aliases: AU045529; Bub1A; BUB1B; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1

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UniProt ID: (Human) O60566, (Mouse) Q9Z1S0

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Entrez Gene ID: (Human) 701, (Mouse) 12236

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

protein phosphorylation apoptotic process sister chromatid cohesion mitotic nuclear division metaphase/anaphase transition of mitotic cell cycle mitotic cell cycle checkpoint mitotic spindle assembly checkpoint cell proliferation anaphase-promoting complex-dependent catabolic process protein localization to kinetochore protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition cell cycle phosphorylation protein localization to chromosome, centromeric region

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BubR1 Polyclonal Antibody

BubR1 Antibody (11504-2-AP) in ICC/IF

Product Details

11504-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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