Invitrogen

C/EBP alpha Monoclonal Antibody (15C8)

13 Published Figures
10 References
View all (24) C/EBP alpha antibodies

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Datasheet
Protocols
Questions & Answers

Product Details

MA1-825

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
View 2 publications 2 publications

ChIP assay (ChIP)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15C8

Immunogen

Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2077903

Product Specific Information

MA1-825 detects C/EBP alpha protein in human, mouse and rat samples.

MA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45 kDa protein representing C/EBP alpha in transfected hu293T cells. MA1-825 is specific to the amino terminal region of C/EBP alpha.

The MA1-825 immunogen is a synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha. This peptide (Cat. # PEP-242) is available for use in neutralization and control experiments.

Target Information

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C EBP; C/EBP alpha; CAAT/enhancer-binding protein DNA-binding protein; CAAT/enhancer-binding protein, DNA-binding protein; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer binding protein, alpha; CCAAT/enhancer-binding protein alpha; OTTHUMP00000220269

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Gene Aliases: C/EBP-alpha; C/ebpalpha; CBF-A; CEBP; CEBPA; DBPCEP

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UniProt ID: (Human) P49715, (Mouse) P53566, (Rat) P05554

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Entrez Gene ID: (Human) 1050, (Mouse) 12606, (Rat) 24252

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding kinase binding protein homodimerization activity transcription regulatory region DNA binding RNA polymerase I regulatory region DNA binding protein domain specific binding protein complex binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity HMG box domain binding
Process(es)
urea cycle negative regulation of transcription from RNA polymerase II promoter liver development embryonic placenta development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase I promoter transcription from RNA polymerase II promoter mitochondrion organization Notch signaling pathway multicellular organism development cholesterol metabolic process negative regulation of cell proliferation cytokine-mediated signaling pathway myeloid cell differentiation macrophage differentiation lung development granulocyte differentiation regulation of cell proliferation glucose homeostasis fat cell differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase III promoter cell maturation inner ear development white fat cell differentiation brown fat cell differentiation lipid homeostasis cellular response to lithium ion cellular response to organic cyclic compound generation of precursor metabolites and energy viral process positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity acute-phase response response to nutrient cell differentiation organ regeneration response to vitamin B2 response to glucocorticoid
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