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Invitrogen
C/EBP delta/epsilon Polyclonal Antibody
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FIGURE: 1 / 4
C/EBP delta/epsilon Antibody (PA5-99597) in ICC/IF
Immunofluorescent analysis of C/EBP delta/epsilon in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with C/EBP delta/epsilon polyclonal antibody (Product # PA5-99597) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-99597
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CEBPD(Accession P49716), corresponding to amino acid residues K184-H234.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818530
Product Specific Information
Antibody detects endogenous levels of total C/EBP delta/epsilon.
Target Information
C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic α helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C EBP; C/EBP delta; C/EBP epsilon; C/EBP-related protein 1; C/EBP-related protein 3; C/EBPepsilon; CCAAT/enhancer binding protein (C/EBP), delta; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer binding protein epsilon; CCAAT/enhancer-binding protein delta; CCAAT/enhancer-binding protein epsilon; NF-IL6-beta; Nuclear factor NF-IL6-beta
Gene Aliases: C/EBP-delta; C/EBP-epsilon; C/EBPe; CEBPD; CEBPE; CELF; CRP1; CRP3; Gm294; NF-IL6-beta
UniProt ID: (Human) P49716, (Human) Q15744, (Mouse) Q00322, (Mouse) Q6PZD9
Entrez Gene ID: (Human) 1052, (Human) 1053, (Mouse) 12609, (Mouse) 110794
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
protein homodimerization activity
protein heterodimerization activity
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
hematopoietic progenitor cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
fat cell differentiation
positive regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
phagocytosis
defense response
macrophage differentiation
cytokine biosynthetic process
defense response to bacterium
cellular response to lipopolysaccharide
positive regulation of gene expression
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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