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Invitrogen
C. botulinum BoNT-B Light Chain Polyclonal Antibody
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C. botulinum BoNT-B Light Chain Antibody (PA5-47737) in WB
Toxins 2022 -
Figure 4 Uptake of BoNT/B-MY but not wild-type BoNT/B into SIMA-hPOMC1-26-Gluc cells. SIMA-hPOMC1-26-Gluc cells were cultured and differentiated, as detailed in the methods section and then incubated with 1 nM BoNT/B or BoNT/B-MY for the times indicated. ( A ) The concentration of the BoNT/B light chain was determined by Western blot in the medium at 0 h (input, Ip) and in cell lysates after 1, 24, and 48 h... View More
Product Details
PA5-47737
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant Clostridium BoNT-B Light Chain Pro2-His428
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610692
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant Light Chains of BoNT-A, -E, or recombinant Heavy Chains of BoNT-C, or -D is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction. E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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