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Invitrogen
C. tetani TeNT Light Chain Monoclonal Antibody (604023)
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C. tetani TeNT Light Chain Antibody (MA5-24273) in WB
Western blot analysis of C. tetani TeNT Light Chain in recombinant C. tetani TeNT Light Chain. Samples were incubated in C. tetani TeNT Light Chain monoclonal antibody (Product # MA5-24273) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for TeNT Light Chain at approximately 53 kDa (as indicated). This experiment was conducted under re... View More
Product Details
MA5-24273
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
604023
Immunogen
E. coli-derived recombinant C. tetani TeNT Light Chain Pro2-Gly430
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610622
Product Specific Information
In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length. The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long. The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation. Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln- -Phe-77’ bond of synaptobrevin-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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