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Invitrogen
C14orf179 Polyclonal Antibody
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FIGURE: 1 / 5
C14orf179 Antibody (PA5-144009) in ICC/IF
Immunocytochemistry analysis of C14orf179 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in C14orf179 Polyclonal antibody (Product # PA5-144009) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and inc... View More
Product Details
PA5-144009
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human IFT43 recombinant protein (Position: M1-T208).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075223
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human U-87MG whole cell, human T-47D whole cell, human MCF-7 whole cell, human PC-3 whole cell, human A549 whole cell, human Jurkat whole cell, human A431 whole cell, rat testis tissue, rat kidney tissue, rat liver tissue, mouse testis tissue, mouse kidney tissue, mouse liver tissue. ICC/IF: T-47D cell. Flow: Caco-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IFT complex A subunit; intraflagellar transport 43 homolog; Intraflagellar transport protein 43 homolog
Gene Aliases: 1700019E19Rik; C14orf179; CED3; IFT43; R75064; RGD1307392
UniProt ID: (Human) Q96FT9, (Mouse) Q9DA69
Entrez Gene ID: (Human) 112752, (Mouse) 76411, (Rat) 299209
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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