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Invitrogen
C1orf74 Polyclonal Antibody
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FIGURE: 1 / 2
C1orf74 Antibody (PA5-31680) in ICC/IF
C1orf74 Polyclonal Antibody detects C1orf74 protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C1orf74 protein stained by C1orf74 Polyclonal Antibody (Product # PA5-31680) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-31680
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human C1orf74. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549153
Product Specific Information
Recommended positive controls: U87-MG.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
C1orf74, also known as URCL4, is located on chromosome 1q32.2 and encodes a previously uncharacterized protein. Recent studies have revealed that C1orf74 is significantly up-regulated in various cancers, including cervical cancer and lung adenocarcinoma. The overexpression of C1orf74 is associated with poor prognosis, indicating its potential role in tumor progression and proliferation. Mechanistically, C1orf74 has been shown to positively regulate the EGFR/AKT/mTORC1 signaling pathway, which is crucial for cell growth and survival. Knockdown studies have demonstrated that reducing C1orf74 expression inhibits cancer cell growth, highlighting it as a potential therapeutic target. Additionally, C1orf74's role in immune cell infiltration suggests it may influence the tumor immune microenvironment, further implicating its importance in cancer biology.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: up-regulated in lung cancer 4; UPF0739 protein C1orf74
Gene Aliases: C1orf74; URLC4
UniProt ID: (Human) Q96LT6
Entrez Gene ID: (Human) 148304
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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