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C1orf77 Antibody (PA5-44307) in IHC
Immunofluorescent analysis of C1orf77 (red) and Insulin (green) were performed on mouse pancreatic tissue from NOD (Non-obese diabetic) mice and CD1 (cluster of differentiation 1) mice. To expose target proteins, antigen retrieval was performed by microwaving tissues for three times with each 5 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval slides were wash with PBS and PBS+1% lamb serum, and then the slides were blocked with 1:50 normal donkey serum for 30 min at room temperature. The tissues were probed for C... View More
Product Details
PA5-44307
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide directed towards the middle region of human C1orf77
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576376
Product Specific Information
Peptide sequence: LKQSLKQRLG KSNIQARLGR PIGALARGAI GGRGLPIIQR GLPRGGLRGG
Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
PA5-44307 detects C1orf77 in IF application with mouse samples.
Target Information
CHTOP is a small nuclear protein that is characterized by an arginine and glycine rich region. This protein may have an important role in the regulation of fetal globin gene expression and in the activation of estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastomagenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chromatin target of PRMT1 protein; DKFZP547E1010; Friend of Prmt1; Friend of PRMT1 protein; RP1-178F15.2; Small arginine- and glycine-rich protein; small protein rich in arginine and glycine; SRAG
Gene Aliases: 2500003M10Rik; C1orf77; CHTOP; FL-SRAG; FOP; HT031; MNCb-1706; PP7704; SRAG; SRAG-3; SRAG-5
UniProt ID: (Human) Q9Y3Y2, (Mouse) Q9CY57
Entrez Gene ID: (Human) 26097, (Mouse) 66511
transcription, DNA-templated
regulation of transcription, DNA-templated
termination of RNA polymerase II transcription
RNA export from nucleus
mRNA export from nucleus
positive regulation of cell proliferation
positive regulation of histone methylation
mRNA 3'-end processing
positive regulation of ATPase activity
positive regulation of helicase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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