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Product Details
61019PROGEN
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
H206
Immunogen
Human Complement Component C3
Conjugate
Form
Lyophilized
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute lyophilized product with 1 mL distilled water.
H 206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c). Disorders specifically detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b and negative control anti-C3a. Activated, deposited C3b bears C3b-a and C3 but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with H 13. Polypeptide reacting: C3b alpha-chain. This antibody was used in a Indirect ELISA.
Target Information
Inactive complement component 3b (iC3b) is a 42kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; iC3b; prepro-C3
Gene Aliases: AHUS5; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p
UniProt ID: (Human) P01024
Entrez Gene ID: (Human) 718
positive regulation of type IIa hypersensitivity
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
tolerance induction
proteolysis
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
blood coagulation
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
negative regulation of endopeptidase activity
regulation of complement activation
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of developmental growth
regulation of immune response
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
positive regulation of apoptotic cell clearance
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