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C4orf23 Antibody (PA5-144043) in WB
Western blot analysis of TRMT44 using TRMT44 antibody (Product # PA5-144043). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human K562 whole cell lysates; Lane 2: human MOLT-4 whole cell lysates; Lane 3: human U251 whole cell lysates; Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. B... View More
Product Details
PA5-144043
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TRMT44 recombinant protein (Position: M1-S757).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075257
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human MOLT-4 whole cell, human U251 whole cell, human Hela whole cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Probable adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: methyltransferase like 19; Methyltransferase-like protein 19; Probable tRNA (uracil-O(2)-)-methyltransferase
Gene Aliases: C4orf23; METTL19; TRM44; TRMT44
UniProt ID: (Human) Q8IYL2
Entrez Gene ID: (Human) 152992
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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