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Progen
C5b neo Monoclonal Antibody (HCC5b.1(neo))
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Product Details
61022PROGEN
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Goat,
Human,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
HCC5b.1(neo)
Immunogen
Activated Human Complement Component C5
Conjugate
Form
Lyophilized
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute lyophilized product with 1 mL distilled water.
HCC 5b.1(neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5!Polypeptide reacting: MW 200 000 polypeptide of SDS denaturated complement component C5 and C5b of MW 185 000; after C5b activation, an MW 60 000 cleavage product also reacts (weakly)Disorders specifically detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1. This antibody was used in a Indirect ELISA.
Target Information
C5b-9 is also known as the TCC. TCC consists of C5b, C6, C7, C8 and C9. The complex forms transmembrane channels which displace lipid molecules and other constituents, thus disrupting the phospholipid bilayer of target cells leading to cell lysis by osmotic leakage. The complex contains neoantigens that are absent from the individual native components and the monoclonal antibody DIA 011-01 is directed against a neoepitope exposed in C9 when incorporated into TCC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5a anaphylatoxin; CHE1; Complement C5; complement C5a anaphylatoxin; complement component C5; FLJ17816; FLJ17822; MGC142298; prepro-C5
Gene Aliases: C5; C5a; C5b; C5D; CPAMD4; ECLZB
UniProt ID: (Human) P01031
Entrez Gene ID: (Human) 727, (Bovine) 512045, (Pig) 414437
activation of MAPK activity
in utero embryonic development
chemotaxis
response to stress
inflammatory response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
positive regulation of vascular endothelial growth factor production
negative regulation of macrophage chemotaxis
negative regulation of endopeptidase activity
cytolysis
regulation of complement activation
positive regulation of angiogenesis
cell chemotaxis
positive regulation of chemokine secretion
mitophagy in response to mitochondrial depolarization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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