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CACNA1B (CaV2.2) Antibody (ACC-002-200UL) in ICC/IF
Expression of CaV2.2 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. A. Cells were stained using Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:200) followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C.... View More
Product Details
ACC-002-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat CACNA1B, Intracellular loop between domains II and III
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The expression of multiple classes of voltage dependent calcium channels (VDCCs) allows neurons to tailor calcium signaling to functionally discrete cellular regions. Although N-Type VDCCs exist before birth which is consistent with a role in migration, most N-Type VDCCs subunit expression is postnatal and is important in the genesis of synaptic transmission in discrete hippocampal fields.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BIII; Brain calcium channel III; calcium channel alpha12.2 subunit; calcium channel, L type, alpha-1 polypeptide; Calcium channel, L type, alpha-1 polypeptide isoform 5; calcium channel, voltage-dependent, L type, alpha 1B subunit; calcium channel, voltage-dependent, N type, alpha 1B subunit; Cav2.2 voltage-gated Ca2+ channel; voltage gated N-type calcium channel Ca(v)2.2; Voltage-dependent N-type calcium channel subunit alpha-1B; voltage-gated calcium channel alpha subunit Cav2.2; Voltage-gated calcium channel subunit alpha Cav2.2
Gene Aliases: alpha(1B); AW050276; AW060892; AW822256; BIII; CACH5; CACNA1B; CACNL1A5; CACNN; Cav2.2; Cchn1a; DYT23
UniProt ID: (Human) Q00975, (Mouse) O55017
Entrez Gene ID: (Human) 774, (Mouse) 12287, (Rat) 257648
voltage-gated calcium channel activity
calcium channel activity
calcium ion binding
protein binding
ATP binding
protein C-terminus binding
high voltage-gated calcium channel activity
nucleotide binding
ion channel activity
voltage-gated ion channel activity
metal ion binding
protein phosphatase 2A binding
transport
chemical synaptic transmission
neurotransmitter secretion
locomotory behavior
regulation of heart contraction
regulation of blood pressure
regulation of ion transmembrane transport
response to pain
membrane depolarization
regulation of calcium ion transport
calcium ion transmembrane transport
membrane depolarization during action potential
positive regulation of neurotransmitter secretion
ion transport
calcium ion transport
response to organic cyclic compound
sensory perception of pain
response to testosterone
transmembrane transport
calcium ion import
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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