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CACNA2D3 Antibody (PA5-87802) in WB
Western blot analysis of CACNA2D3 in extracts of various cell lines. Samples were incubated with CACNA2D3 Polyclonal antibody (Product # PA5-87802) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87802
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 900-1040 of human CACNA2D3 (NP_0608682)
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804420
Product Specific Information
Immunogen sequence: LYDYQAMCRA NKESSDGAHG LLDPYNAFLS AVKWIMTELV LFLVEFNLCS WWHSDMTAKA QKLKQTLEPC DTEYPAFVSE RTIKETTGNI ACEDCSKSFV IQQIPSSNLF MVVVDSSCLC ESVAPITMAP IEIRYNESLK C; Positive Samples: U-87 MG, HeLa, Hep G2, Mouse brain, Rat brain; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
CACNA2D3 is a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 2 delta-3; alpha2delta3; calcium channel alpha2-delta3 subunit; calcium channel, voltage dependent, alpha2/delta subunit 3; calcium channel, voltage-dependent, alpha 2/delta 3 subunit; calcium channel, voltage-dependent, alpha 2/delta subunit 3; CaV alpha2delta3; Voltage-dependent calcium channel subunit alpha-2/delta-3; Voltage-gated calcium channel subunit alpha-2/delta-3
Gene Aliases: CACNA2D3; Cacnad3; HSA272268
UniProt ID: (Human) Q8IZS8, (Rat) Q8CFG5, (Mouse) Q9Z1L5
Entrez Gene ID: (Human) 55799, (Rat) 306243, (Mouse) 12294
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