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CADM3 Antibody (PA5-96305) in WB
Western blot analysis of CADM3 in extracts of various cell lines. Samples were incubated with CADM3 Polyclonal antibody (Product # PA5-96305) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96305
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-330 of human CADM3 (NP_0011206451)
Conjugate
Form
Liquid
Concentration
1.31 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808107
Product Specific Information
Immunogen sequence: NLSQDDSQPW TSDETVVAGG TVVLKCQVKD HEDSSLQWSN PAQQTLYFGE KRALRDNRIQ LVTSTPHELS ISISNVALAD EGEYTCSIFT MPVRTAKSLV TVLGIPQKPI ITGYKSSLRE KDTATLNCQS SGSKPAARLT WRKGDQELHG EPTRIQEDPN GKTFTVSSSV TFQVTREDDG ASIVCSVNHE SLKGADRSTS QRIEVLYTPT AMIRPDPPHP REGQKLLLHC EGRGNPVPQQ YLWEKEGSVP PLKMTQESAL IFPFLNKSDS GTYGCTATSN MGSYKAYYTL NVNDPSPVPS SSSTYH; Positive Samples: U-87MG, MCF-7, HL-60, Mouse brain, Rat brain; Cellular Location: Cell junction, Cell membrane, Single-pass type I membrane protein
Target Information
IGSF4B is a brain-specific protein related to the calcium-independent cell-cell adhesion molecules known as nectins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Brain immunoglobulin receptor; Cell adhesion molecule 3; dendritic cell nectin-like protein 1 short isoform; IgSF4B; Immunoglobulin superfamily member 4B; immunoglobulin superfamily, member 4B; NECL-1; nectin-like 1; Nectin-like protein 1; nectin-lke 1; Synaptic cell adhesion molecule 3; SynCAM3; TSLC1-like 1; TSLC1-like protein 1; TSLL1
Gene Aliases: BIgR; CADM3; IGSF4B; Necl-1; NECL1; SYNCAM3; TSLL1; UNQ225/PRO258
UniProt ID: (Human) Q8N126, (Mouse) Q99N28, (Rat) Q1WIM3
Entrez Gene ID: (Human) 57863, (Mouse) 94332, (Rat) 360882
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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