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Invitrogen
CAR Polyclonal Antibody
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FIGURE: 1 / 4
CAR Antibody (PA5-95066) in WB
Western blot analysis of CAR in Lane 1: human A431 whole cell lysates, Lane 2: human HeLa whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U-937whole cell lysates (negative), Lane 6: human THP-1whole cell lysates (negative). Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with CAR p... View More
Product Details
PA5-95066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Coxsackie Adenovirus Receptor (313-328aa YSKTQYNQVPSEDFER).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806872
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, rat heart tissue, mouse liver tissue, mouse HEPA1-6 whole cell, human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell, human U-937whole cell(negative), human THP-1whole cell(negative).|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; Coxsackievirus and adenovirus receptor; Coxsackievirus and adenovirus receptor homolog; Coxsackievirus B-adenovirus receptor; CVB3-binding protein; HCVADR; rCAR
Gene Aliases: 2610206D03Rik; AU016810; AW553441; CAR; Car1; CAR4/6; CXADR; HCAR; MCAR; MCVADR
UniProt ID: (Human) P78310, (Mouse) P97792, (Rat) Q9R066
Entrez Gene ID: (Human) 1525, (Mouse) 13052, (Rat) 89843
mitochondrion organization
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
heart development
germ cell migration
epithelial structure maintenance
neutrophil chemotaxis
actin cytoskeleton reorganization
cell-cell junction organization
gamma-delta T cell activation
viral entry into host cell
cardiac muscle fiber development
regulation of immune response
leukocyte migration
transepithelial transport
AV node cell to bundle of His cell communication
cell adhesion
single organismal cell-cell adhesion
homotypic cell-cell adhesion
defense response to virus
negative regulation of cardiac muscle cell proliferation
positive regulation of epithelial cell proliferation involved in wound healing
AV node cell-bundle of His cell adhesion involved in cell communication
regulation of AV node cell action potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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