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CARF Antibody (A303-862A) in WB
Detection of human CARF by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (H; 50 µg), and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CARF antibody (Product # A303-862A) (lot A303-862A-1) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-862A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 225 and 275 of human Collaborator of ARF.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDKN2A-interacting protein; collaborates/cooperates with ARF (alternate reading frame) protein; Collaborator of ARF
Gene Aliases: CARF; CDKN2AIP
UniProt ID: (Human) Q9NXV6
Entrez Gene ID: (Human) 55602
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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