Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Bethyl Laboratories
CARMIL2 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
CARMIL2 Antibody (A305-831A-T) in WB
Detection of human and mouse CARMIL2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, MCF-7, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARMIL2 antibody A305-831A-M (lot A305-831A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A305-831A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 1385 and 1435 of human Leucine-rich repeat-containing protein 16C.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
RLTPR/CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR/CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR/CARMIL2 is crucial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR/CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR/CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Capping protein regulator and myosin 1 linker 2; Capping protein, Arp2/3 and myosin-I linker protein 2; F-actin-uncapping protein RLTPR; leucine rich repeat containing 16C; Leucine-rich repeat-containing protein 16C; RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; RGD, leucine-rich repeat, tropomodulin and proline-rich containing protein; RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein
Gene Aliases: CARMIL2; CARMIL2b; CG1399-PB; D130029J02Rik; Gm585; LRRC16C; RLTPR
UniProt ID: (Human) Q6F5E8
Entrez Gene ID: (Human) 146206, (Mouse) 234695
establishment or maintenance of cell polarity
positive regulation of lamellipodium assembly
maintenance of cell polarity
positive regulation of cell migration
positive regulation of T cell proliferation
wound healing, spreading of cells
establishment of protein localization
positive regulation of regulatory T cell differentiation
thymus development
homeostasis of number of cells
T cell receptor signaling pathway
actin filament network formation
establishment or maintenance of monopolar cell polarity
positive regulation of extracellular matrix disassembly
positive regulation of ruffle assembly
positive regulation of interleukin-2 secretion
positive regulation of interferon-gamma secretion
positive regulation of lamellipodium organization
negative regulation of barbed-end actin filament capping
cell migration
positive regulation of interferon-gamma production
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support