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CASK Antibody (PA1-544) in ICC/IF
Immunofluorescent analysis of CASK was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CASK Rabbit Polyclonal Antibody (Product # PA1-544) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
Product Details
PA1-544
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C(578) E R D S P S T S R Q S P A N(592)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2243867
Product Specific Information
PA1-544 detects calmodulin sensitive kinase (CASK) from mouse and rat tissues and cells.
PA1-544 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with PA1-544 results in staining of the choroid plexus.
PA1-544 immunizing peptide corresponds to amino acid residues 578-592 from human CASK. This sequence is completely conserved between rat, mouse, and human CASK.
PA1-544 can be used with blocking peptide PEP-110.
Target Information
CASK is a calcium/calmodulin-dependent serine protein kinase which is a MAGUK (membrane-associated guanylate kinase) protein family member. CASK is a scaffold protein and the encoded protein is located at synapses in the brain. CASK associates with FG syndrome 4, mental retardation, microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. CASK functions as a cytoskeletal membrane scaffold that coordinates signal transduction pathways within the cortical cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium/calmodulin-dependent serin protein kinase; Calcium/calmodulin-dependent serine protein kinase; calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase; hCASK; MGC7449; OTTHUMP00000023157; OTTHUMP00000023161; OTTHUMP00000023162; Peripheral plasma membrane protein CASK; Protein lin-2 homolog; RP23-278L4.1; trinucleotide repeat containing 8
Gene Aliases: CAGH39; CAMGUK; CASK; CMG; DXPri1; DXRib1; FGS4; LIN-2; LIN2; MICPCH; mLin-2; MRXSNA; Pals3; TNRC8
UniProt ID: (Human) O14936, (Rat) Q62915, (Mouse) O70589
Entrez Gene ID: (Human) 8573, (Rat) 29647, (Mouse) 12361
guanylate kinase activity
protein serine/threonine kinase activity
protein binding
calmodulin binding
ATP binding
neurexin family protein binding
transcription coactivator activity
calmodulin-dependent protein kinase activity
G-protein coupled receptor activity
protein C-terminus binding
PDZ domain binding
protein complex binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
negative regulation of cell-matrix adhesion
protein complex assembly
protein phosphorylation
intracellular protein transport
G-protein coupled receptor signaling pathway
negative regulation of keratinocyte proliferation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of wound healing
calcium ion import
positive regulation of calcium ion import
negative regulation of cellular response to growth factor stimulus
regulation of nucleic acid-templated transcription
phosphorylation
cell adhesion
neurotransmitter secretion
GMP metabolic process
GDP metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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