Invitrogen

CBF beta Polyclonal Antibody

5 Published Figures

5 References

View all (20) CBF beta antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CBF beta Antibody (PA1-317) in WB

Western blot detection of CBF beta from HeLa whole cell lysates using Product # PA1-317.

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Product Details

PA1-317

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2070590

Product Specific Information

PA1-317 detects CBF-beta in human samples.

PA1-317 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates.

The PA1-317 immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta. This sequence is conserved in mouse. PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.

Target Information

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression.

Disease markers

Hsu CH,Ma HP,Ong JR,Hsieh MS,Yadav VK,Yeh CT,Chao TY,Lee WH,Huang WC,Kuo KT,Fong IH,Lin CC,Su CM

PA1-317 was used in Western Blotting to reveale if core binding factor subunit β may promote bone metastasis in patients with breast cancer.

Mon Aug 01 00:00:00 UTC 2022

Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive Binding of Core-Binding Factor Beta.

Journal of virology

Miyagi E,Welbourn S,Sukegawa S,Fabryova H,Kao S,Strebel K

PA1-317 was used in Western Blotting to identified CBFβ as the key player involved in D/N interference by Vif.

Tue Mar 17 00:00:00 UTC 2020

CBFβ enhances de novo protein biosynthesis of its binding partners HIV-1 Vif and RUNX1 and potentiates the Vif-induced degradation of APOBEC3G.

Journal of virology

Miyagi E,Kao S,Yedavalli V,Strebel K

PA1-317 was used in western blot to study the mechanisms underlying the effects of CBF-beta on the synthesis of HIV1 Vif and cellular RUNX and on the degradation of APOBEC3G mediated by Vif

Thu May 01 00:00:00 UTC 2014

A stable transcription factor complex nucleated by oligomeric AML1-ETO controls leukaemogenesis.

Nature

Sun XJ,Wang Z,Wang L,Jiang Y,Kost N,Soong TD,Chen WY,Tang Z,Nakadai T,Elemento O,Fischle W,Melnick A,Patel DJ,Nimer SD,Roeder RG

PA1-317 was used in western blot to study the role of AML1-ETO complex formation in the mechanism by which this transcription factor promotes leukemogenesis

Thu Aug 01 00:00:00 UTC 2013

Novel function of the unique N-terminal region of RUNX1c in B cell growth regulation.

Nucleic acids research

Brady G,Elgueta Karstegl C,Farrell PJ

PA1-317 was used in western blot to study the role of the N-terminal sequence of RUNX1c in regulating B-cell growth

Fri Feb 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CBF-beta; Core-binding factor subunit beta; PEA2-beta; PEBP2-beta; polyomavirus enhancer binding protein 2, beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit

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Gene Aliases: CBFB; PEBP2B

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UniProt ID: (Human) Q13951

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Entrez Gene ID: (Human) 865

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity transcription coactivator activity protein binding

Process(es)

protein polyubiquitination osteoblast differentiation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter lymphocyte differentiation myeloid cell differentiation positive regulation of transcription from RNA polymerase II promoter cell maturation definitive hemopoiesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CBF beta Polyclonal Antibody

CBF beta Antibody (PA1-317) in WB

Product Details

PA1-317

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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