Invitrogen

CBP Polyclonal Antibody

9 Published Figures

12 References

View all (18) CBP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (9)

CBP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

CBP Antibody (PA1-847) in ICC/IF

Immunofluorescent analysis of CREB Binding Protein (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incub... View More

CBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CBP Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-847

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2083939

Product Specific Information

PA1-847 detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) from human and mouse tissues and cells.

PA1-847 has been successfully used in ChIP, ICC/IF, Western blot and IHC-P procedures. By Western blot, this antibody detects an ~265 kDa protein representing CBP and a larger, unknown protein at ~400 kDa from HeLa cell lysate.

The PA1-847 immunogen is a synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. The PA1-847 immunizing peptide (Cat. # PEP-052) is available for use in neutralization and control experiments.

Target Information

CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Purine-Induced IFN-γ Promotes Uric Acid Production by Upregulating Xanthine Oxidoreductase Expression.

Frontiers in immunology

Wang H,Xie L,Song X,Wang J,Li X,Lin Z,Su T,Liang B,Huang D

PA1-847 was used in ChIP assay to explore whether exogenous purines are responsible for increased xanthine oxidoreductase expression and activity.

Thu Mar 03 00:00:00 UTC 2022

Purine-Induced IFN-γ Promotes Uric Acid Production by Upregulating Xanthine Oxidoreductase Expression.

Frontiers in immunology

Wang H,Xie L,Song X,Wang J,Li X,Lin Z,Su T,Liang B,Huang D

PA1-847 was used in ChIP assay to explore whether exogenous purines are responsible for increased xanthine oxidoreductase expression and activity.

Thu Mar 03 00:00:00 UTC 2022

Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia.

eLife

Takao S,Forbes L,Uni M,Cheng S,Pineda JMB,Tarumoto Y,Cifani P,Minuesa G,Chen C,Kharas MG,Bradley RK,Vakoc CR,Koche RP,Kentsis A

PA1-847 was used in Western Blot to conclude that aberrant assembly and sequestration of MYB:CBP/P300 complexes provide a unifying mechanism of oncogenic gene expression in AML.

Tue Feb 02 00:00:00 UTC 2021

Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia.

eLife

Takao S,Forbes L,Uni M,Cheng S,Pineda JMB,Tarumoto Y,Cifani P,Minuesa G,Chen C,Kharas MG,Bradley RK,Vakoc CR,Koche RP,Kentsis A

PA1-847 was used in Western Blot to conclude that aberrant assembly and sequestration of MYB:CBP/P300 complexes provide a unifying mechanism of oncogenic gene expression in AML.

Tue Feb 02 00:00:00 UTC 2021

LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.

Cell death & disease

Xie X,Lin J,Fan X,Zhong Y,Chen Y,Liu K,Ren Y,Chen X,Lai D,Li X,Li Z,Tang A

Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Fri Feb 19 00:00:00 UTC 2021

Aberrant activation of CYR61 enhancers in colorectal cancer development.

Journal of experimental & clinical cancer research : CR

Xie L,Song X,Lin H,Chen Z,Li Q,Guo T,Xu T,Su T,Xu M,Chang X,Wang LK,Liang B,Huang D

PA1-847 was used in Western Blotting to study the mechanism of action of cysteine-rich 61 in the regulation of transcription in colorectal cancer.

Wed May 22 00:00:00 UTC 2019

Peptidomimetic blockade of MYB in acute myeloid leukemia.

Nature communications

Ramaswamy K,Forbes L,Minuesa G,Gindin T,Brown F,Kharas MG,Krivtsov AV,Armstrong SA,Still E,de Stanchina E,Knoechel B,Koche R,Kentsis A

Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Tue Jan 09 00:00:00 UTC 2018

Expression of glial CBP in steroid mediated neuroprotection in male and female zebra finches.

Journal of chemical neuroanatomy

Klores M,Moon JT,Duncan KA

PA1847 was used in immunohistochemistry to explore the role of cAMP response element-binding protein-binding protein in the estrogen and immune response to injury

Sun Jan 01 00:00:00 UTC 2017

Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2.

Molecular endocrinology (Baltimore, Md.)

Hahm JB,Privalsky ML

PA1-847 was used in Western Blotting to identify a series of previously unrecognized proteins that selectively interact with thyroid hormone receptorΒ2, compared with the more widely expressed TRΒ1.

Wed May 01 00:00:00 UTC 2013

Cells in behaviourally relevant brain regions coexpress nuclear receptor coactivators and ovarian steroid receptors.

Journal of neuroendocrinology

Tetel MJ,Siegal NK,Murphy SD

PA1-847 was used in immunohistochemistry and western blot to investigate the expression of nuclear receptor coactivators and ovarian steroid receptors in particular brain regions

Sun Apr 01 00:00:00 UTC 2007

Cells in behaviourally relevant brain regions coexpress nuclear receptor coactivators and ovarian steroid receptors.

Journal of neuroendocrinology

Tetel MJ,Siegal NK,Murphy SD

PA1-847 was used in immunohistochemistry and western blot to investigate the expression of nuclear receptor coactivators and ovarian steroid receptors in particular brain regions

Sun Apr 01 00:00:00 UTC 2007

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats.

Hormones and behavior

Molenda-Figueira HA,Williams CA,Griffin AL,Rutledge EM,Blaustein JD,Tetel MJ

PA1-847 was used in western blot to study the ER and PR action regulated by SRC-1 and CBP in the rat brain

Fri Sep 01 00:00:00 UTC 2006

Nuclear receptor coactivators modulate hormone-dependent gene expression in brain and female reproductive behavior in rats.

Endocrinology

Molenda HA,Griffin AL,Auger AP,McCarthy MM,Tetel MJ

PA1-847 was used in immunocytochemistry to demonstrate the regulatory role of nuclear receptors in ER transcriptional activity in brain and the expression of hormone-dependent behavior.

Fri Feb 01 00:00:00 UTC 2002

The transcriptional coactivators p300 and CBP are histone acetyltransferases.

Cell

Ogryzko VV,Schiltz RL,Russanova V,Howard BH,Nakatani Y

PA1-847 was used in western blot to investigate the functional properties of CREB binding protein and p300

Fri Nov 29 00:00:00 UTC 1996

Bioinformatics

Protein Aliases: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein lactyltransferas CREBBP; Protein-lysine acetyltransferase CREBBP

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Gene Aliases: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS

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UniProt ID: (Human) Q92793

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Entrez Gene ID: (Human) 1387, (Mouse) 12914

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Function(s)

core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcriptional repressor activity, RNA polymerase II transcription factor binding p53 binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity histone acetyltransferase activity signal transducer activity protein binding transcription factor binding zinc ion binding acetyltransferase activity peptide N-acetyltransferase activity peroxisome proliferator activated receptor binding MRF binding SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription cofactor activity transferase activity transferase activity, transferring acyl groups activating transcription factor binding metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein acetylation cell proliferation histone acetylation N-terminal peptidyl-lysine acetylation germ-line stem cell population maintenance cellular response to UV positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of G1/S transition of mitotic cell cycle response to hypoxia stimulatory C-type lectin receptor signaling pathway transcription initiation from RNA polymerase II promoter protein complex assembly signal transduction Notch signaling pathway regulation of smoothened signaling pathway viral process positive regulation of type I interferon production homeostatic process embryonic digit morphogenesis regulation of apoptotic process cellular lipid metabolic process positive regulation of cell adhesion molecule production regulation of transcription from RNA polymerase II promoter in response to hypoxia response to interleukin-1 cell-cell adhesion regulation of cellular response to heat positive regulation of NIK/NF-kappaB signaling beta-catenin-TCF complex assembly

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CBP Polyclonal Antibody

CBP Antibody (PA1-847) in ICC/IF

Product Details

PA1-847

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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