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Invitrogen
CBP Polyclonal Antibody
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CBP Antibody (PA5-27369) in ICC/IF
Immunofluorescence analysis of CBP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CBP Polyclonal Antibody (Product # PA5-27369) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution... View More
Product Details
PA5-27369
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human CBP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.35 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544845
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT-116, Vero E6.
Predicted reactivity: Mouse (94%), Rat (94%), Rhesus Monkey (100%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein lactyltransferas CREBBP; Protein-lysine acetyltransferase CREBBP
Gene Aliases: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS
UniProt ID: (Human) Q92793
Entrez Gene ID: (Human) 1387, (Mouse) 12914
core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
transcriptional repressor activity, RNA polymerase II transcription factor binding
p53 binding
chromatin binding
damaged DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
histone acetyltransferase activity
signal transducer activity
protein binding
transcription factor binding
zinc ion binding
acetyltransferase activity
peptide N-acetyltransferase activity
peroxisome proliferator activated receptor binding
MRF binding
SMAD binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription cofactor activity
transferase activity
transferase activity, transferring acyl groups
activating transcription factor binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein acetylation
cell proliferation
histone acetylation
N-terminal peptidyl-lysine acetylation
germ-line stem cell population maintenance
cellular response to UV
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of G1/S transition of mitotic cell cycle
response to hypoxia
stimulatory C-type lectin receptor signaling pathway
transcription initiation from RNA polymerase II promoter
protein complex assembly
signal transduction
Notch signaling pathway
regulation of smoothened signaling pathway
viral process
positive regulation of type I interferon production
homeostatic process
embryonic digit morphogenesis
regulation of apoptotic process
cellular lipid metabolic process
positive regulation of cell adhesion molecule production
regulation of transcription from RNA polymerase II promoter in response to hypoxia
response to interleukin-1
cell-cell adhesion
regulation of cellular response to heat
positive regulation of NIK/NF-kappaB signaling
beta-catenin-TCF complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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