GenScript

CBP-tag Polyclonal Antibody

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CBP-tag Antibody (A00635-40) in WB

Western blot analysis of CBP tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-CBP-tag Polyclonal Antibody (GenScript, A00635, 1 µg/mL)The signal was developed with IRDy TM 800 Conjugated Goat Anti-Rabbit IgG.

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Product Details

A00635-40

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

ELISA (ELISA)

0.05-0.2 µg/mL

Immunoprecipitation (IP)

1-5 µg/mL

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.GenScript Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH. CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The antibody is suitable for detecting CBP fusion proteins.

Target Information

CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PAXIP1 Potentiates the Combination of WEE1 Inhibitor AZD1775 and Platinum Agents in Lung Cancer.

Molecular cancer therapeutics

Jhuraney A,Woods NT,Wright G,Rix L,Kinose F,Kroeger JL,Remily-Wood E,Cress WD,Koomen JM,Brantley SG,Gray JE,Haura EB,Rix U,Monteiro AN

The DNA damage response (DDR) involves a complex network of signaling events mediated by modular protein domains such as the BRCA1 C-terminal (BRCT) domain. Thus, proteins that interact with BRCT domains and are a part of the DDR constitute potential targets for sensitization to DNA-damaging chemotherapy agents. We performed a pharmacologic screen to evaluate 17 kinases, identified in a BRCT-mediated interaction network as targets to enhance platinum-based chemotherapy in lung cancer. Inhibition

Fri Jul 01 00:00:00 UTC 2016

Promoter sequences direct cytoplasmic localization and translation of mRNAs during starvation in yeast.

Nature

Zid BM,O'Shea EK

A universal feature of the response to stress and nutrient limitation is transcriptional upregulation of genes that encode proteins important for survival. Under many such conditions, the overall protein synthesis level is reduced, thereby dampening the stress response at the level of protein expression. For example, during glucose starvation in Saccharomyces cerevisiae (yeast), translation is rapidly repressed, yet the transcription of many stress- and glucose-repressed genes is increased. Here

Thu Oct 02 00:00:00 UTC 2014

A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast.

Molecular and cellular biology

Yu Y,Srinivasan M,Nakanishi S,Leatherwood J,Shilatifard A,Sternglanz R

A screen of Saccharomyces cerevisiae histone alanine substitution mutants revealed that mutations in any of three adjacent residues, L97, Y98, or G99, near the C terminus of H4 led to a unique phenotype. The mutants grew slowly, became polyploid or aneuploid rapidly, and also lost chromosomes at a high rate, most likely because their kinetochores were not assembled properly. There was lower histone occupancy, not only in the centromeric region, but also throughout the genome for the H4 mutants.

Wed Jun 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CREB binding protein (Rubinstein-Taybi syndrome); CREB-binding protein

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Gene Aliases: AW558298; BOS_22444; CBP; CBP/p300; hmm291030; KAT3A; p300/CBP; RSTS; RTS

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Entrez Gene ID: (Bovine) 505453, (Mouse) 12914, (Rat) 54244

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity protein binding transcription factor binding zinc ion binding acetyltransferase activity transferase activity transferase activity, transferring acyl groups activating transcription factor binding peroxisome proliferator activated receptor binding MRF binding SMAD binding metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein acetylation cell proliferation histone acetylation N-terminal peptidyl-lysine acetylation germ-line stem cell population maintenance cellular response to UV positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of G1/S transition of mitotic cell cycle response to interleukin-1

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CBP-tag Polyclonal Antibody

CBP-tag Antibody (A00635-40) in WB

Product Details

A00635-40

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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