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Invitrogen
CBX4 Recombinant Rabbit Monoclonal Antibody (23GB1905)
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FIGURE: 1 / 3
CBX4 Antibody (MA5-53154) in ICC/IF
Immunocytochemistry analysis of CBX4 in HepG2 cells. Nuclei were stained blue with DAPI; CBX4 recombinant monoclonal antibody (Product # MA5-53154) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Product Details
MA5-53154
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB1905
Immunogen
A synthesized peptide derived from human CBX4 (500-560AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3247627
Target Information
Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chromobox homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox protein homolog 4; chromobox-like protein 4; E3 SUMO-protein ligase CBX4; E3 SUMO-protein transferase CBX4; hPc2; NS5ATP1-binding protein 16; Pc class 2 homolog; Pc2; Polycomb 2 homolog
Gene Aliases: CBX4; MPc2; NBP16; PC2
UniProt ID: (Human) O00257, (Mouse) O55187
Entrez Gene ID: (Human) 8535, (Mouse) 12418, (Rat) 501403
chromatin binding
transcription corepressor activity
single-stranded RNA binding
protein binding
ligase activity
SUMO transferase activity
enzyme binding
SUMO binding
methylated histone binding
transcription regulatory region DNA binding
phosphoprotein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
covalent chromatin modification
protein sumoylation
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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