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Invitrogen
CCKAR Polyclonal Antibody
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CCKAR Antibody (PA3-116) in ICC/IF
Immunofluorescence analysis of CCK-A Receptor was performed using 70% confluent log phase PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CCKAR Rabbit Polyclonal Antibody (Product # PA3-116) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conju... View More
Product Details
PA3-116
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, KLH conjugated, corresponding to the residues T(409) G A S L S R F S Y S H M S A S V P P Q (428) of human CCK1 receptor.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2072495
Product Specific Information
PA3-116 detects CCK1-Receptor in human samples.
PA3-116 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~80-90 kDa protein representing CCK1-Receptor in human samples. In immunohistochemistry, PAC1 immunoreactivity is predominantly localized at the plasma membrane and in cells of the gastric mucosa, myenteric ganglion cells, myenteric nerve fibers in the gasrtointestinal tract.
PA3-116 immunogen is a synthetic peptide, KLH conjugated, corresponding to the residues T(409) G A S L S R F S Y S H M S A S V P P Q (428) of human VPAC2 receptor.
Target Information
This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCK-A receptor; CCK-AR; CCK1-R; ccka receptor; Cholecystokinin receptor type A; cholecystokinin type-A receptor; Cholecystokinin-1 receptor; HGNC:1570
Gene Aliases: CCK-A; CCK1-R; CCK1R; CCKAR; CCKRA
UniProt ID: (Human) P32238
Entrez Gene ID: (Human) 886
temperature homeostasis
gastric acid secretion
neuron migration
reduction of food intake in response to dietary excess
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
axonogenesis
response to nutrient
digestion
feeding behavior
response to radiation
response to heat
insulin secretion
pancreatic juice secretion
forebrain development
pancreas development
organ regeneration
actin cytoskeleton reorganization
response to lipopolysaccharide
cellular response to hormone stimulus
cholecystokinin signaling pathway
response to starvation
regulation of potassium ion transport
regulation of hormone secretion
response to glucocorticoid
regulation of calcium ion transport
positive regulation of somatostatin secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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