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CCL16 Antibody (500-P125GBT-25UG) in WB
Western Blot: To detect hLEC by Western Blot analysis CCL16 Polyclonal Antibody, Biotin (Product # 500-P125GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P125GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human LEC (CCL16)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929358
Product Specific Information
AA Sequence of recombinant protein: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human LEC (CCL16). Anti-Human LEC (CCL16)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human LEC (CCL16) (500-P125G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LEC (CCL16).
Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P125GBT-1MG will be provided as 2 x 500 µg
Target Information
CCL16 gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL16 displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 16; chemokine (C-C motif) ligand 16; Chemokine CC-4; Chemokine LEC; HCC-4; IL-10-inducible chemokine; LCC-1; liver CC chemokine-1; Liver-expressed chemokine; LMC; Lymphocyte and monocyte chemoattractant; MGC117051; Monotactin-1; MTN-1; NCC-4; new CC chemokine 4; small inducible cytokine subfamily A (Cys-Cys), member 16; Small-inducible cytokine A16
Gene Aliases: CCL16; CKb12; HCC-4; ILINCK; LCC-1; LEC; LMC; Mtn-1; NCC-4; NCC4; SCYA16; SCYL4
UniProt ID: (Human) O15467
Entrez Gene ID: (Human) 6360
monocyte chemotaxis
chemotaxis
inflammatory response
cell communication
G-protein coupled receptor signaling pathway
cell-cell signaling
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
positive chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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