Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Rockland
CCL2 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
CCL2 Antibody (200-401-B60) in WB
Western blot using Rocklands protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Inc. Anti-bovine CCL2. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (611-743-127).
Product Details
200-401-B60
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Sus scrofa 85.5% and Bos Taurus 81.1%. Cross-reactivity with CCL2 from other sources has not been determined.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Acidic seminal fluid protein; C-C motif chemokine 2; H-MCP-1; MCP-1; MGC9434; monocyte chemoattractant protein 1; monocyte chemotactic protein 1; Monocyte chemotactic protein 1A; RP23-350G1.3; Small-inducible cytokine A2
Gene Aliases: BOS_18463; CCL2; MCP-1; MCP-1A; MCP1; MCP1A; SCYA2
UniProt ID: (Bovine) P28291
Entrez Gene ID: (Bovine) 281043
MAPK cascade
monocyte chemotaxis
inflammatory response
cytoskeleton organization
regulation of cell shape
regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
negative regulation of angiogenesis
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
helper T cell extravasation
negative regulation of apoptotic process
protein kinase B signaling
astrocyte cell migration
cellular response to fibroblast growth factor stimulus
macrophage chemotaxis
positive regulation of T cell activation
positive regulation of nitric-oxide synthase biosynthetic process
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to organic cyclic compound
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support