Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), Functional Grade, eBioscience™

6 Published Figures
10 References
View all (67) CCL2 (MCP-1) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

16-7096-81

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

-
View 3 publications 3 publications

Neutralization (Neu)

Assay-Dependent
View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H5

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469220

Product Specific Information

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. .

Applications Tested: The Functional Grade purified 2H5 antibody has been tested by ELISA for verification of reactivity and by LAL assay for verification of low endotoxin. The 2H5 antibody has been published for use in vitro and in vivo for neutralization of MCP-1 activity.

Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2

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Gene Aliases: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296

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Function(s)
protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding G-protein coupled receptor binding cytokine activity protein binding
Process(es)
MAPK cascade response to hypoxia positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response monocyte chemotaxis positive regulation of leukocyte migration positive regulation of cellular extravasation cellular calcium ion homeostasis inflammatory response cytoskeleton organization transforming growth factor beta receptor signaling pathway aging glial cell migration regulation of cell shape response to heat response to wounding response to mechanical stimulus response to bacterium response to gamma radiation regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis response to activity negative regulation of angiogenesis cytokine-mediated signaling pathway positive regulation of cell-cell adhesion neutrophil chemotaxis organ regeneration lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to progesterone positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of collagen biosynthetic process response to vitamin B3 negative regulation of glial cell apoptotic process helper T cell extravasation cellular response to drug cellular response to macrophage colony-stimulating factor stimulus cellular response to platelet-derived growth factor stimulus chemokinesis response to drug response to amino acid protein kinase B signaling negative regulation of neuron apoptotic process astrocyte cell migration cellular response to fibroblast growth factor stimulus response to ethanol positive regulation of cell adhesion response to antibiotic vascular endothelial growth factor receptor signaling pathway macrophage chemotaxis lymphocyte chemotaxis positive regulation of synaptic transmission positive regulation of T cell activation response to glucocorticoid positive regulation of nitric-oxide synthase biosynthetic process maternal process involved in female pregnancy maternal process involved in parturition chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to ATP cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to lipoprotein particle stimulus cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of calcium ion import positive regulation of protein targeting to membrane positive regulation of apoptotic cell clearance negative regulation of natural killer cell chemotaxis angiogenesis protein phosphorylation chemotaxis humoral immune response cell adhesion signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger JAK-STAT cascade organ morphogenesis viral genome replication cellular homeostasis PERK-mediated unfolded protein response positive regulation of GTPase activity positive regulation of inflammatory response positive regulation of ERK1 and ERK2 cascade immune response monocyte homeostasis eosinophil chemotaxis
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