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CCL21 Antibody (500-P114-1MG) in WB
Western Blot: To detect Murine Exodus-2 (CCL21) by Western Blot analysis CCL21 Polyclonal Antibody (Product # 500-P114-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P114-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine Exodus-2 (CCL21)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929276
Product Specific Information
AA Sequence of recombinant protein: SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Exodus-2 (CCL21). Anti-Murine Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography employing an immobilized Murine Exodus-2 (CCL21) matrix.
Sandwich ELISA: To detect Murine Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine Exodus-2 (CCL21) (500-P114Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Exodus-2 (CCL21).
Western Blot: To detect Murine Exodus-2 (CCL21) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6 Ckine; 6Ckine; beta chemokine exodus-2; Beta-chemokine exodus-2; C-C motif chemokine 21a; C-C motif chemokine 21b; C-C motif chemokine 21c; CC chemokine 6Ckine-ser; Ccl21-leu; CCL21-Ser; exodus-2; secondary lymphoid organ chemokine; small inducible cytokine A21; small inducible cytokine A21a; small inducible cytokine A21c; Small-inducible cytokine A21a; Small-inducible cytokine A21b; Small-inducible cytokine A21c; TCA4; Thymus-derived chemotactic agent 4
Gene Aliases: 6CKBAC1; 6CKBAC2; 6Ckine; AI159701; ALP; AW987545; Ccl21a; Ccl21b; Ccl21c; CKb9; exodus-2; plt; Scya21; SCYA21a; Scya21b; Scya21c; SLC; TCA4
UniProt ID: (Mouse) P84444, (Mouse) P86792
Entrez Gene ID: (Mouse) 18829, (Mouse) 100042493, (Mouse) 65956
Performance Guarantee
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